Anti-TNFSF10 Antibody (CAB2138)
- SKU:
- CAB2138
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-TNFSF10 Antibody |
| Antibody SKU: | CAB2138 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 39-250 of human TNFSF10 (NP_003801.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 39-250 of human TNFSF10 (NP_003801.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TNEL KQMQ DKYS KSGI ACFL KEDD SYWD PNDE ESMN SPCW QVKW QLRQ LVRK MILR TSEE TIST VQEK QQNI SPLV RERG PQRV AAHI TGTR GRSN TLSS PNSK NEKA LGRK INSW ESSR SGHS FLSN LHLR NGEL VIHE KGFY YIYS QTYF RFQE EIKE NTKN DKQM VQYI YKYT SYPD PILL MKSA RNSC WSKD AEYG LYSI YQGG IFEL |
| Gene ID: | 8743 |
| Uniprot: | P50591 |
| Cellular Location: | Membrane, Single-pass type II membrane protein |
| Calculated MW: | 11kDa/32kDa |
| Observed MW: | 28kDa |
| Synonyms: | TNFSF10, APO2L, Apo-2L, CD253, TL2, TNLG6A, TRAIL |
| Background: | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. |
| NCBI Summary: | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P50591 |
| NCBI GenInfo Identifier: | 1730015 |
| NCBI Gene ID: | 8743 |
| NCBI Accession: | P50591.1 |
| UniProt Secondary Accession: | P50591,A1Y9B3, |
| UniProt Related Accession: | P50591 |
| Molecular Weight: | 11,746 Da |
| NCBI Full Name: | Tumor necrosis factor ligand superfamily member 10 |
| NCBI Synonym Full Names: | TNF superfamily member 10 |
| NCBI Official Symbol: | TNFSF10 |
| NCBI Official Synonym Symbols: | TL2; APO2L; CD253; TRAIL; Apo-2L; TNLG6A |
| NCBI Protein Information: | tumor necrosis factor ligand superfamily member 10 |
| UniProt Protein Name: | Tumor necrosis factor ligand superfamily member 10 |
| UniProt Synonym Protein Names: | Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253 |
| UniProt Gene Name: | TNFSF10 |
 | Western blot analysis of extracts of mouse kidney, using TNFSF10 antibody (CAB2138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Western blot analysis of extracts of various cell lines, using TNFSF10 antibody (CAB2138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using TNFSF10 antibody (CAB2138) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human prostate using TNFSF10 antibody (CAB2138) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using TNFSF10 antibody (CAB2138) at dilution of 1:200 (40x lens). |
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