Anti-TNFR1 Antibody (CAB1540)

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SKU:
CAB1540
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Frequently bought together:

Description

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Antibody Name:Anti-TNFR1 Antibody
Antibody SKU:CAB1540
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-211 of human TNFR1 (NP_001056.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-211 of human TNFR1 (NP_001056.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IYPS GVIG LVPH LGDR EKRD SVCP QGKY IHPQ NNSI CCTK CHKG TYLY NDCP GPGQ DTDC RECE SGSF TASE NHLR HCLS CSKC RKEM GQVE ISSC TVDR DTVC GCRK NQYR HYWS ENLF QCFN CSLC LNGT VHLS CQEK QNTV CTCH AGFF LREN ECVS CSNC KKSL ECTK LCLP QIEN VKGT EDSG TT
Gene ID:7132
Uniprot:P19438
Cellular Location:Cell membrane, Golgi apparatus membrane, Secreted, Secreted, Single-pass type I membrane protein
Calculated MW:24kDa/25kDa/38kDa/50kDa
Observed MW:55KDa
Synonyms:TNFRSF1A, CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60
Background:This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients.
UniProt Protein Function:TNF-R1: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein. Interacts with human cytomegalovirus/HHV-5 protein UL138. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: Golgi membrane; extracellular space; cell surface; mitochondrion; integral to plasma membrane; axon; extracellular region; plasma membrane; synapse; nucleus; cytosol; receptor complex; lipid raft

Molecular Function:protein binding; tumor necrosis factor receptor activity; protease binding; protein complex binding; tumor necrosis factor binding

Biological Process: response to alkaloid; viral reproduction; protein heterooligomerization; response to lipopolysaccharide; tumor necrosis factor-mediated signaling pathway; positive regulation of neuron apoptosis; negative regulation of interleukin-6 production; response to wounding; tetrapyrrole metabolic process; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein import into nucleus, translocation; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; response to amino acid stimulus; prostaglandin metabolic process; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of angiogenesis; response to ethanol; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of inflammatory response; defense response to bacterium; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; positive regulation of inflammatory response; negative regulation of apoptosis

Disease: Multiple Sclerosis, Susceptibility To, 5; Periodic Fever, Familial, Autosomal Dominant

NCBI Summary:The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq, Jul 2008]
UniProt Code:P19438
NCBI GenInfo Identifier:135959
NCBI Gene ID:7132
NCBI Accession:P19438.1
UniProt Secondary Accession:P19438,Q9UCA4, A8K4X3, B2RDE4, B3KPQ1, B4DQB7, B4E309 B5M0B5, D3DUR1,
UniProt Related Accession:P19438
Molecular Weight:24,194 Da
NCBI Full Name:Tumor necrosis factor receptor superfamily member 1A
NCBI Synonym Full Names:tumor necrosis factor receptor superfamily, member 1A
NCBI Official Symbol:TNFRSF1A
NCBI Official Synonym Symbols:FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
NCBI Protein Information:tumor necrosis factor receptor superfamily member 1A; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor-alpha receptor; tumor necrosis factor receptor type 1; tumor necrosis factor binding protein 1; tumor necrosis factor receptor 1A isoform beta
UniProt Protein Name:Tumor necrosis factor receptor superfamily member 1A
UniProt Synonym Protein Names:Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD_antigen: CD120aCleaved into the following 2 chains:Tumor necrosis factor receptor superfamily member 1A, membrane form; Tumor necrosis factor-binding protein 1; TBPI
UniProt Gene Name:TNFRSF1A
UniProt Entry Name:TNR1A_HUMAN
Anti-TNFR1 Antibody (CAB1540)Western blot analysis of extracts of various cell lines, using TNFR1 antibody (CAB1540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00035). Exposure time: 90s.
Anti-TNFR1 Antibody (CAB1540)Immunohistochemistry of paraffin-embedded rat lung using TNFR1 Rabbit pAb (CAB1540) at dilution of 1:100 (40x lens).
Anti-TNFR1 Antibody (CAB1540)Immunohistochemistry of paraffin-embedded rat spleen using TNFR1 Rabbit pAb (CAB1540) at dilution of 1:100 (40x lens).
Anti-TNFR1 Antibody (CAB1540)Immunohistochemistry of paraffin-embedded mouse spleen using TNFR1 Rabbit pAb (CAB1540) at dilution of 1:100 (40x lens).
Anti-TNFR1 Antibody (CAB1540)Immunofluorescence analysis of BALB-3T3 cells using TNFR1 Rabbit pAb (CAB1540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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