Anti-TMEM74 Antibody (CAB10379)
- SKU:
- CAB10379
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-TMEM74 Antibody |
| Antibody SKU: | CAB10379 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TMEM74 (NP_694560.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | DU145 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human TMEM74 (NP_694560.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MELH YLAK KSNQ ADLC DARD WSSR GLPG DQAD TAAT RAAL CCQK QCAS TPRA TEME GSKL SSSP ASPS SSLQ NSTL QPDA FPPG LLHS GNNQ ITAE RKVC NCCS QELE TSFT YVDK NINL EQRN RSSP SAKG HNHP GELG WENP NEWS QEAA ISLI SEEE DDTS SEAT SSGK SIDY GFIS |
| Gene ID: | 157753 |
| Uniprot: | Q96NL1 |
| Cellular Location: | Cytoplasmic vesicle, Lysosome membrane, Multi-pass membrane protein, autophagosome membrane |
| Calculated MW: | 33kDa |
| Observed MW: | 37kDa |
| Synonyms: | TMEM74, NET36 |
| Background: |
| UniProt Protein Function: | TMEM74: Plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. Belongs to the TMEM74 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 8q23.1 Molecular Function:protein binding Biological Process: macroautophagy |
| UniProt Code: | Q96NL1 |
| NCBI GenInfo Identifier: | 74732587 |
| NCBI Gene ID: | 157753 |
| NCBI Accession: | Q96NL1.1 |
| UniProt Related Accession: | Q96NL1 |
| Molecular Weight: | 33kDa |
| NCBI Full Name: | Transmembrane protein 74 |
| NCBI Synonym Full Names: | transmembrane protein 74 |
| NCBI Official Symbol: | TMEM74 |
| NCBI Official Synonym Symbols: | NET36 |
| NCBI Protein Information: | transmembrane protein 74 |
| UniProt Protein Name: | Transmembrane protein 74 |
| Protein Family: | Transmembrane protein |
| UniProt Gene Name: | TMEM74 |
 | Western blot analysis of extracts of DU145 cells, using TMEM74 antibody (CAB10379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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