Anti-TM9SF1 Antibody (CAB7461)
- SKU:
- CAB7461
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-TM9SF1 Antibody |
| Antibody SKU: | CAB7461 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-230 of human TM9SF1 (NP_006396.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NCI-H460, U-251MG, SW620, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-230 of human TM9SF1 (NP_006396.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PGVE GVTH YKAG DPVI LYVN KVGP YHNP QETY HYYQ LPVC CPEK IRHK SLSL GEVL DGDR MAES LYEI RFRE NVEK RILC HMQL SSAQ VEQL RQAI EELY YFEF VVDD LPIR GFVG YMEE SGFL PHSH KIGL WTHL DFHL EFHG DRII FANV SVRD VKPH SLDG LRPD EFLG LTHT YSVR WSET SVER RSDR RRGD DGGF FPR |
| Gene ID: | 10548 |
| Uniprot: | O15321 |
| Cellular Location: | Cytoplasmic vesicle, Lysosome membrane, Multi-pass membrane protein, autophagosome membrane |
| Calculated MW: | 55kDa/68kDa |
| Observed MW: | 80kDa |
| Synonyms: | TM9SF1, HMP70, MP70 |
| Background: |
| UniProt Protein Function: | TM9SF1: Plays an essential role in autophagy. Belongs to the nonaspanin (TM9SF) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: lysosomal membrane; integral to membrane; cytoplasmic vesicle Biological Process: autophagy |
| UniProt Code: | O15321 |
| NCBI GenInfo Identifier: | 62299047 |
| NCBI Gene ID: | 10548 |
| NCBI Accession: | O15321.2 |
| UniProt Secondary Accession: | O15321,Q86SZ6, Q96FI8, D3DS65, |
| UniProt Related Accession: | O15321 |
| Molecular Weight: | 606 |
| NCBI Full Name: | Transmembrane 9 superfamily member 1 |
| NCBI Synonym Full Names: | transmembrane 9 superfamily member 1 |
| NCBI Official Symbol: | TM9SF1 |
| NCBI Official Synonym Symbols: | MP70; HMP70 |
| NCBI Protein Information: | transmembrane 9 superfamily member 1; MP70 protein family member; multispanning membrane protein (70kD); transmembrane protein 9 superfamily member 1 |
| UniProt Protein Name: | Transmembrane 9 superfamily member 1 |
| UniProt Synonym Protein Names: | MP70 protein family member; hMP70 |
| Protein Family: | Transmembrane 9 superfamily |
| UniProt Gene Name: | TM9SF1 |
| UniProt Entry Name: | TM9S1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TM9SF1 antibody (CAB7461) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using TM9SF1 antibody (CAB7461) at dilution of 1:100 (40x lens). |
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