Anti-TJP2 Antibody (CAB0594)
- SKU:
- CAB0594
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TJP2 Antibody |
| Antibody SKU: | CAB0594 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 951-1190 of human TJP2 (NP_004808.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, Jurkat, Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 951-1190 of human TJP2 (NP_004808.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RSSE PVQH EESI RKPS PEPR AQMR RAAS SDQL RDNS PPPA FKPE PPKA KTQN KEES YDFS KSYE YKSN PSAV AGNE TPGA STKG YPPP VAAK PTFG RSIL KPST PIPP QEGE EVGE SSEE QDNA PKSV LGKV KIFE KMDH KARL QRMQ ELQE AQNA RIEI AQKH PDIY AVPI KTHK PDPG TPQH TSSR PPEP QKAP SRPY QDTR GSYG SDAE EEEY RQQL SEHS KRGY YGQS ARYR DTEL |
| Gene ID: | 9414 |
| Uniprot: | Q9UDY2 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasmic side, Nucleus, Peripheral membrane protein, adherens junction, tight junction |
| Calculated MW: | 111-137kDa |
| Observed MW: | 160kDa |
| Synonyms: | TJP2, C9DUPq21.11, DFNA51, DUP9q21.11, PFIC4, X104, ZO2 |
| Background: | This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. |
| UniProt Protein Function: | ZO2: a scaffolding protein of the MAGUK (membrane-associated guanylate kinase) family associated with epithelial tight junctions (TJ). May mediate dynamic changes in the composition of TJs. Plays a role in tight junctions and adherens junctions. Forms homodimers, and heterodimer with ZO-1 and ZO-3. The ZOs shuttle between the TJ and the nucleus, where they may regulate gene expression. Interacts with occluding, UBN1 and scribble. Interacts with SAFB in the nucleus. TJ, or zonula occludens, are the closely associated areas of two cells whose membranes are tightly joined together. They are composed of a branching network of strands, the efficiency of which increases exponentially with the number of strands. The strands associate with transmembrane proteins that bind to similar proteins on adjacent cells, and with submembranous proteins that are anchored to the actin component of the cytoskeleton. Thus, TJs join together the cytoskeletons of adjacent cells. They endow tissues with substantial form, shape and location, enforce cellular polarity, and form barriers to molecules and pathogens. Molecules forming the membranous part of TJs include occludin, claudins, tricellulin and junctional adhesion molecules. These molecules interact with the proteins ZO-1, ZO-2 and ZO-3. Tight junction proteins are involved in the epithelial-mesenchymal transitions (EMT) in tumors. Five isoforms of the human protein are produced by alternative promoters. Isoform A1 is abundant in the heart and brain. Detected in brain and skeletal muscle. It is present almost exclusively in normal tissues. Isoform C1 is expressed at high level in the kidney, pancreas, heart and placenta. Not detected in brain and skeletal muscle. Found in normal as well as in most neoplastic tissues.. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 9q13-q21 Cellular Component: nucleoplasm; adherens junction; tight junction; cytoplasm; plasma membrane; cytosol; cell junction Molecular Function:protein binding, bridging; protein C-terminus binding; protein domain specific binding; guanylate kinase activity; protein binding Biological Process: response to organic substance; apoptosis; nucleotide phosphorylation; intestinal absorption; cell structure disassembly during apoptosis Disease: Hypercholanemia, Familial; Cholestasis, Progressive Familial Intrahepatic, 4 |
| NCBI Summary: | This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
| UniProt Code: | Q9UDY2 |
| NCBI GenInfo Identifier: | 317373313 |
| NCBI Gene ID: | 9414 |
| NCBI Accession: | Q9UDY2.2 |
| UniProt Secondary Accession: | Q9UDY2,Q15883, Q5VXL0, Q5VXL1, Q8N756, Q8NI14, Q99839 A2A3H9, B7Z2R8, B7Z7T6, F5H301, F5H886, |
| UniProt Related Accession: | Q9UDY2 |
| Molecular Weight: | 1190 |
| NCBI Full Name: | Tight junction protein ZO-2 |
| NCBI Synonym Full Names: | tight junction protein 2 |
| NCBI Official Symbol: | TJP2 |
| NCBI Official Synonym Symbols: | ZO2; X104; PFIC4; DFNA51; DUP9q21.11; C9DUPq21.11 |
| NCBI Protein Information: | tight junction protein ZO-2; zona occludens 2; zonula occludens protein 2; Friedreich ataxia region gene X104 (tight junction protein ZO-2) |
| UniProt Protein Name: | Tight junction protein ZO-2 |
| UniProt Synonym Protein Names: | Tight junction protein 2; Zona occludens protein 2; Zonula occludens protein 2 |
| Protein Family: | Tight junction protein |
| UniProt Gene Name: | TJP2 |
| UniProt Entry Name: | ZO2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TJP2 antibody (CAB0594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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