Anti-TIMP3 Antibody (CAB11986)
- SKU:
- CAB11986
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-TIMP3 Antibody |
| Antibody SKU: | CAB11986 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human TIMP3 (NP_000353.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, 293T, A431, mouse kidney |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human TIMP3 (NP_000353.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YQYL LTGR VYDG KMYT GLCN FVER WDQL TLSQ RKGL NYRY HLGC NCKI KSCY YLPC FVTS KNEC LWTD MLSN FGYP GYQS KHYA CIRQ KGGY CSWY RGWA PPDK SIIN ATDP |
| Gene ID: | 7078 |
| Uniprot: | P35625 |
| Cellular Location: | Secreted, extracellular matrix, extracellular space |
| Calculated MW: | 24kDa |
| Observed MW: | 20kDa |
| Synonyms: | TIMP3, HSMRK222, K222, K222TA2, SFD |
| Background: | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. |
| UniProt Protein Function: | TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 22q12.3 Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; basement membrane; nucleus Molecular Function:metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding Biological Process: negative regulation of metalloenzyme activity; visual perception; response to cytokine stimulus; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis Disease: Fundus Dystrophy, Pseudoinflammatory, Of Sorsby |
| NCBI Summary: | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35625 |
| NCBI GenInfo Identifier: | 730948 |
| NCBI Gene ID: | 7078 |
| NCBI Accession: | P35625.2 |
| UniProt Secondary Accession: | P35625,Q5THV4, Q9UC74, Q9UGS2, B2RBY9, |
| UniProt Related Accession: | P35625 |
| Molecular Weight: | 24,145 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 3 |
| NCBI Synonym Full Names: | TIMP metallopeptidase inhibitor 3 |
| NCBI Official Symbol: | TIMP3 |
| NCBI Official Synonym Symbols: | SFD; K222; K222TA2; HSMRK222 |
| NCBI Protein Information: | metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3 |
| UniProt Protein Name: | Metalloproteinase inhibitor 3 |
| UniProt Synonym Protein Names: | Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | TIMP3 |
| UniProt Entry Name: | TIMP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TIMP3 antibody (CAB11986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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