Anti-TCF25 Antibody (CAB15140)
- SKU:
- CAB15140
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-TCF25 Antibody |
| Antibody SKU: | CAB15140 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TCF25 (NP_055787.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TCF25 (NP_055787.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSRR ALRR LRGE QRGQ EPLG PGAL HFDL RDDD DAEE EGPK RELG VRRP GGAG KEGV RVNN RFEL INID DLED DPVV NGER SGCA LTDA VAPG NKGR GQRG NTES KTDG DDTE TVPS EQSH ASGK LRKK KKKQ KNKK SSTG EASE NGLE DIDR ILER IEDS TGLN RPGP APLS SRKH VLYV EHRH LNPD TELK RYFG ARAI LGEQ RPRQ RQRV YPKC TWLT TPKS TWPR YSKP GLSM RLLE SKKG LSFF AFEH SEEY QQAQ HKFL VAVE SMEP NNIV VLLQ TSPY HVDS LLQL SDAC RFQE |
| Gene ID: | 22980 |
| Uniprot: | Q9BQ70 |
| Cellular Location: | Nucleus |
| Calculated MW: | 76kDa |
| Observed MW: | 95kDa |
| Synonyms: | TCF25, FKSG26, Hulp1, NULP1, PRO2620, hKIAA1049 |
| Background: |
| UniProt Protein Function: | TCF25: May play a role in cell death control. Acts as a transcriptional repressor. Has been shown to repress transcription of SRF in vitro and so may play a role in heart development. Belongs to the TCF25 family. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 16q24.3 Cellular Component: nucleus Molecular Function:protein binding; transcription factor activity Biological Process: negative regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm, 2008 [PubMed 18068114]).[supplied by OMIM, Sep 2008] |
| UniProt Code: | Q9BQ70 |
| NCBI GenInfo Identifier: | 23396596 |
| NCBI Gene ID: | 22980 |
| NCBI Accession: | Q9BQ70.1 |
| UniProt Secondary Accession: | Q9BQ70,Q2MK75, Q9UPV3, |
| UniProt Related Accession: | Q9BQ70 |
| Molecular Weight: | 76,667 Da |
| NCBI Full Name: | Transcription factor 25 |
| NCBI Synonym Full Names: | transcription factor 25 |
| NCBI Official Symbol: | TCF25 |
| NCBI Official Synonym Symbols: | Hulp1; NULP1; FKSG26; PRO2620; hKIAA1049 |
| NCBI Protein Information: | transcription factor 25 |
| UniProt Protein Name: | Transcription factor 25 |
| UniProt Synonym Protein Names: | Nuclear localized protein 1 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | TCF25 |
| UniProt Entry Name: | TCF25_HUMAN |
 | Western blot analysis of extracts of U-87MG cells, using TCF25 antibody (CAB15140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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