Anti-TBX5 항체 (CAB15590)

Antibody Name:	Anti-TBX5 Antibody
Antibody SKU:	CAB15590
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TBX5 (NP_852259.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TBX5 (NP_852259.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADA DEGF GLAH TPLE PDAK DLPC DSKP ESAL GAPS KSPS SPQA AFTQ QGME GIKV FLHE RELW LKFH EV
Gene ID:	6910
Uniprot:	Q99593
Cellular Location:	Nucleus
Calculated MW:	39kDa/52kDa/57kDa
Observed MW:	58kDa

Synonyms:

TBX5, HOS, T-box 5

Background:

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

UniProt Protein Function:	TBX5: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Defects in TBX5 are the cause of Holt-Oram syndrome (HOS). HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 12q24.1 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding Biological Process: embryonic forelimb morphogenesis; pericardium development; transcription initiation from RNA polymerase II promoter; heart development; positive regulation of transcription, DNA-dependent; pattern specification process; cardiac muscle cell differentiation; forelimb morphogenesis; negative regulation of cell proliferation; cell-cell signaling; apoptotic nuclear changes; negative regulation of cardiac muscle cell proliferation; positive regulation of cardioblast differentiation; morphogenesis of an epithelium; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell migration; lung development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation Disease: Holt-oram Syndrome
NCBI Summary:	This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99593
NCBI GenInfo Identifier:	12644474
NCBI Gene ID:	6910
NCBI Accession:	Q99593.2
UniProt Related Accession:	Q99593
Molecular Weight:
NCBI Full Name:	T-box transcription factor TBX5
NCBI Synonym Full Names:	T-box transcription factor 5
NCBI Official Symbol:	TBX5
NCBI Official Synonym Symbols:	HOS
NCBI Protein Information:	T-box transcription factor TBX5
UniProt Protein Name:	T-box transcription factor TBX5
Protein Family:	T-box transcription factor
UniProt Gene Name:	TBX5
UniProt Entry Name:	TBX5_HUMAN

Western blot analysis of extracts of various cell lines, using TBX5 antibody (CAB15590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

TBX5 Antibody (PACO12662)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)