Anti-TBCB Antibody (CAB13248)
- SKU:
- CAB13248
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TBCB Antibody |
| Antibody SKU: | CAB13248 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human TBCB (NP_001272.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, 293T, Mouse brain, Mouse heart, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human TBCB (NP_001272.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEVT GVSA PTVT VFIS SSLN TFRS EKRY SRSL TIAE FKCK LELL VGSP ASCM ELEL YGVD DKFY SKLD QEDA LLGS YPVD DGCR IHVI DHSG ARLG EYED VSRV EKYT ISQE AYDQ RQDT VRSF LKRS KLGR YNEE ERAQ QEAE AAQR LAEE KAQA SSIP VGSR CEVR AAGQ SPRR GTVM YVGL TDFK PGYW IGVR YDEP LGKN DGSV NGKR YFEC QAKY GAFV KPAV VTVG DFPE EDYG LDEI |
| Gene ID: | 1155 |
| Uniprot: | Q99426 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 21kDa/27kDa |
| Observed MW: | 37kDa |
| Synonyms: | TBCB, CG22, CKAP1, CKAPI |
| Background: |
| UniProt Protein Function: | TBCB: a cytoskeletal protein that binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Chaperone; Cytoskeletal Chromosomal Location of Human Ortholog: 19q13.11-q13.12 Cellular Component: microtubule cytoskeleton; nucleoplasm; microtubule; cytoplasm Molecular Function:protein binding Biological Process: nervous system development; 'de novo' posttranslational protein folding; cellular protein metabolic process; cell differentiation |
| UniProt Code: | Q99426 |
| NCBI GenInfo Identifier: | 3023518 |
| NCBI Gene ID: | 1155 |
| NCBI Accession: | Q99426.2 |
| UniProt Secondary Accession: | Q99426,O00111, O00674, O14728, Q6FGY5, |
| UniProt Related Accession: | Q99426 |
| Molecular Weight: | 244 |
| NCBI Full Name: | Tubulin-folding cofactor B |
| NCBI Synonym Full Names: | tubulin folding cofactor B |
| NCBI Official Symbol: | TBCB |
| NCBI Official Synonym Symbols: | CG22; CKAP1; CKAPI |
| NCBI Protein Information: | tubulin-folding cofactor B; tubulin-specific chaperone B; cytoskeleton associated protein 1; cytoskeleton-associated protein CKAPI |
| UniProt Protein Name: | Tubulin-folding cofactor B |
| UniProt Synonym Protein Names: | Cytoskeleton-associated protein 1; Cytoskeleton-associated protein CKAPI; Tubulin-specific chaperone B |
| UniProt Gene Name: | TBCB |
| UniProt Entry Name: | TBCB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TBCB antibody (CAB13248) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat brain using TBCB antibody (CAB13248) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human placenta using TBCB antibody (CAB13248) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using TBCB antibody (CAB13248) at dilution of 1:100 (40x lens). |
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