Anti-TBCA Antibody (CAB13050)
- SKU:
- CAB13050
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TBCA Antibody |
| Antibody SKU: | CAB13050 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human TBCA (NP_004598.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:1000 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | OVCAR3, HeLa, 293T, U-87MG, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human TBCA (NP_004598.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADP RVRQ IKIK TGVV KRLV KEKV MYEK EAKQ QEEK IEKM RAED GENY DIKK QAEI LQES RMMI PDCQ RRLE AAYL DLQR ILEN EKDL EEAE EYKE ARLV LDSV KLEA |
| Gene ID: | 6902 |
| Uniprot: | O75347 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 12kDa/15kDa |
| Observed MW: | 13kDa |
| Synonyms: | TBCA |
| Background: | The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TBCA: Tubulin-folding protein; involved in the early step of the tubulin folding pathway. Belongs to the TBCA family. |
| UniProt Protein Details: | Protein type:Chaperone Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: microtubule cytoskeleton; microtubule; cytoplasm; nucleolus Molecular Function:chaperone binding; unfolded protein binding Biological Process: tubulin folding; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; post-chaperonin tubulin folding pathway |
| NCBI Summary: | The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| UniProt Code: | O75347 |
| NCBI GenInfo Identifier: | 6831691 |
| NCBI Gene ID: | 6902 |
| NCBI Accession: | O75347.3 |
| UniProt Related Accession: | O75347 |
| Molecular Weight: | 12,855 Da |
| NCBI Full Name: | Tubulin-specific chaperone A |
| NCBI Synonym Full Names: | tubulin folding cofactor A |
| NCBI Official Symbol: | TBCA |
| NCBI Protein Information: | tubulin-specific chaperone A; CFA; chaperonin cofactor A; TCP1-chaperonin cofactor A; tubulin-folding cofactor A |
| UniProt Protein Name: | Tubulin-specific chaperone A |
| UniProt Synonym Protein Names: | TCP1-chaperonin cofactor A; Tubulin-folding cofactor A; CFA |
| UniProt Gene Name: | TBCA |
| UniProt Entry Name: | TBCA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TBCA antibody (CAB13050) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using TBCA antibody (CAB13050) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using TBCA antibody (CAB13050) at dilution of 1:100 (40x lens). |
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