Anti-TAZ Antibody (CAB8202)
- SKU:
- CAB8202
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | TAZ Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8202 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TAZ (NP_056287.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, SW480 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TAZ (NP_056287.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNPA SAPP PLPP PGQQ VIHV TQDL DTDL EALF NSVM NPKP SSWR KKIL PESF FKEP DSGS HSRQ SSTD SSGG HPGP RLAG GAQH VRSH SSPA SLQL GTGA GAAG SPAQ QHAH LRQQ SYDV TDEL PLPP GWEM TFTA TGQR YFLN HIEK ITTW QDPR KAMN QPLN HMNL HPAV SSTP VPQR SMAV SQPN LVMN HQHQ QQMA PSTL SQQN HPTQ NPPA GLMS MPNA LTTQ QQ |
| Gene ID: | 25937 |
| Uniprot: | Q9GZV5 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 44kDa |
| Observed MW: | 44KDa |
| Synonyms: | WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif, TAZ, WWTR1 |
| Background: |
| UniProt Protein Function: | TAZ: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. Binds to SLC9A3R2 via the PDZ motif at the plasma membrane. Binds to YWHAZ in vivo and in vitro through the phosphoserine-binding motif RSHSSP. Interacts (via coiled-coil domain) with SMAD2 (via MH1 domain), SMAD3 and SMAD4. Interacts with MED15, PAX8 and NKX2-1. Interacts with TEAD1, TEAD2, TEAD3 and TEAD4. Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue. |
| UniProt Protein Details: | Protein type:Oncoprotein; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 3q23-q24 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:protein binding; transcription coactivator activity Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase activity; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell division; transcription initiation from RNA polymerase II promoter |
| UniProt Code: | Q9GZV5 |
| NCBI GenInfo Identifier: | 67462080 |
| NCBI Gene ID: | 25937 |
| NCBI Accession: | Q9GZV5.1 |
| UniProt Secondary Accession: | Q9GZV5,Q8N3P2, Q9Y3W6, D3DNH7, |
| UniProt Related Accession: | Q9GZV5 |
| Molecular Weight: | 44,101 Da |
| NCBI Full Name: | WW domain-containing transcription regulator protein 1 |
| NCBI Synonym Full Names: | WW domain containing transcription regulator 1 |
| NCBI Official Symbol: | WWTR1 |
| NCBI Official Synonym Symbols: | TAZ |
| NCBI Protein Information: | WW domain-containing transcription regulator protein 1 |
| UniProt Protein Name: | WW domain-containing transcription regulator protein 1 |
| UniProt Synonym Protein Names: | Transcriptional coactivator with PDZ-binding motif |
| Protein Family: | WW domain-containing transcription regulator protein |
| UniProt Gene Name: | WWTR1 |
| UniProt Entry Name: | WWTR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TAZ Rabbit pAb (CAB8202) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human gastric using TAZ antibody (CAB8202) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using TAZ antibody (CAB8202) at dilution of 1:200 (40x lens). |
 | Confocal immunofluorescence analysis of U2OS cells using TAZ antibody (CAB8202) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining. |
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