Anti-TARDBP Antibody (CAB1183)
- SKU:
- CAB1183
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TARDBP Antibody |
| Antibody SKU: | CAB1183 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP RIP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TARDBP (NP_031401.1). |
| Application: | WB IHC IF IP RIP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:20 - 1:100 IP 1:50 - 1:100 RIP 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, SW620, HT-29, A-673, Es-2, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TARDBP (NP_031401.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSEY IRVT EDEN DEPI EIPS EDDG TVLL STVT AQFP GACG LRYR NPVS QCMR GVRL VEGI LHAP DAGW GNLV YVVN YPKD NKRK MDET DASS AVKV KRAV QKTS DLIV LGLP WKTT EQDL KEYF STFG EVLM VQVK KDLK TGHS KGFG FVRF TEYE TQVK VMSQ RHMI DGRW CDCK LPNS KQSQ DEPL RSRK VFVG RCTE DMTE DELR EFFS QYGD VMDV FIPK PFRA FAFV TFAD DQIA QSLC GEDL IIKG ISVH ISNA |
| Gene ID: | 23435 |
| Uniprot: | Q13148 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa/44kDa |
| Observed MW: | 42kDa |
| Synonyms: | ALS10, TDP-43, TARDBP, TDP43 |
| Background: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. |
| NCBI Summary: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13148 |
| NCBI GenInfo Identifier: | 20140568 |
| NCBI Gene ID: | 23435 |
| NCBI Accession: | |
| UniProt Related Accession: | Q13148 |
| Molecular Weight: | 44 |
| NCBI Full Name: | TAR DNA-binding protein 43 |
| NCBI Synonym Full Names: | TAR DNA binding protein |
| NCBI Official Symbol: | TARDBP |
| NCBI Official Synonym Symbols: | ALS10; TDP-43 |
| NCBI Protein Information: | TAR DNA-binding protein 43 |
| UniProt Protein Name: | |
| UniProt Gene Name: |
 | Western blot analysis of extracts of various cell lines, using TARDBP antibody (CAB1183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human esophagus using TARDBP Antibody (CAB1183) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human endometrium using TARDBP Antibody (CAB1183) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using TARDBP antibody (CAB1183). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug TARDBP antibody (CAB1183). Western blot was performed from the immunoprecipitate using TARDBP antibody (CAB1183) at a dilition of 1:500. |
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