Anti-TAF12 Antibody (CAB18073)[KO Validated]

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SKU:
CAB18073
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-TAF12 Antibody [KO Validated]
Antibody SKU:CAB18073
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human TAF12 (NP_001128690.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNQF GPSA LINL SNFS SIKP EPAS TPPQ GSMA NSTA VVKI PGTP GAGG RLSP ENNQ VLTK KKLQ DLVR EVDP NEQL DEDV EEML LQIA DDFI ESVV TAAC QLAR HRKS STLE VKDV QLHL ERQW NMWI PGFG SEEI RPYK KACT TEAH KQRM ALIR KTTK K
Gene ID:6883
Uniprot:Q16514
Cellular Location:Nucleus
Calculated MW:14kDa/17kDa
Observed MW:18kDa
Anti-TAF12 Antibody [KO Validated] (CAB18073)Western blot analysis of extracts from normal (control) and TAF12 knockout (KO) HeLa cells, using TAF12 antibody (CAB18073) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Synonyms:TAF12, TAF2J, TAFII20
Background:Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:TAF12: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. Belongs to the TAF12 family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:

Protein type:Transcription initiation complex

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; transcription factor TFIID complex; PCAF complex

Molecular Function:protein binding; histone acetyltransferase activity; DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription initiation; RNA elongation from RNA polymerase II promoter; positive regulation of transcription factor activity; gene expression

NCBI Summary:Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008]
UniProt Code:Q16514
NCBI GenInfo Identifier:3024708
NCBI Gene ID:6883
NCBI Accession:Q16514.1
UniProt Secondary Accession:Q16514,Q15775, Q5T077, D3DPM5,
UniProt Related Accession:Q16514
Molecular Weight:161
NCBI Full Name:Transcription initiation factor TFIID subunit 12
NCBI Synonym Full Names:TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
NCBI Official Symbol:TAF12  
NCBI Official Synonym Symbols:TAF2J; TAFII20  
NCBI Protein Information:transcription initiation factor TFIID subunit 12; TAFII20/TAFII15; TAFII-20/TAFII-15; transcription initiation factor TFIID 20/15 kDa subunits; TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD
UniProt Protein Name:Transcription initiation factor TFIID subunit 12
UniProt Synonym Protein Names:Transcription initiation factor TFIID 20/15 kDa subunits; TAFII-20/TAFII-15; TAFII20/TAFII15
Protein Family:Transcription initiation factor
UniProt Gene Name:TAF12  
UniProt Entry Name:TAF12_HUMAN

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