Anti-Synaptotagmin-11 Antibody (CAB8198)
- SKU:
- CAB8198
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | SYT11 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8198 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11 (NP_689493.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11 (NP_689493.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | WSCC HQQA EKKQ KNPP YKFI HMLK GISI YPET LSNK KKII KVRR DKDG PGRE GGRR NLLV DAAE AGLL SRDK DPRG PSSG SCID QLPI KMDY GEEL RSPI TSLT PGES KTTS PSSP EEDV MLGS LTFS VDYN FPKK ALVV TIQE AHGL PVMD DQTQ GSDP YIKM |
| Gene ID: | 23208 |
| Uniprot: | Q9BT88 |
| Cellular Location: | Cell junction, Cytoplasmic vesicle, Membrane, Single-pass membrane protein, secretory vesicle, synapse, synaptic vesicle membrane |
| Calculated MW: | 48kDa |
| Observed MW: | 45kDa |
| Synonyms: | SYT11, SYT12, sytXI |
| Background: | This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that are known calcium sensors and mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. The encoded protein is also a substrate for ubiquitin-E3-ligase parkin. The gene has previously been referred to as synaptotagmin XII but has been renamed synaptotagmin XI to be consistent with mouse and rat official nomenclature. |
| UniProt Protein Function: | SYT11: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: synaptic vesicle; synaptic vesicle membrane; integral to plasma membrane; cell junction Molecular Function:protein binding; calcium-dependent phospholipid binding; transporter activity; calcium ion binding Biological Process: transport; negative regulation of neurotransmitter secretion |
| NCBI Summary: | This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that are known calcium sensors and mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. The encoded protein is also a substrate for ubiquitin-E3-ligase parkin. The gene has previously been referred to as synaptotagmin XII but has been renamed synaptotagmin XI to be consistent with mouse and rat official nomenclature. [provided by RefSeq, Apr 2010] |
| UniProt Code: | Q9BT88 |
| NCBI GenInfo Identifier: | 15489165 |
| NCBI Gene ID: | 23208 |
| NCBI Accession: | AAH13690.1 |
| UniProt Secondary Accession: | Q9BT88,Q14998, Q5W0D4, Q68CT5, Q8IXU3, Q96SU2, |
| UniProt Related Accession: | Q9BT88 |
| Molecular Weight: | 48,297 Da |
| NCBI Full Name: | Synaptotagmin XI |
| NCBI Synonym Full Names: | synaptotagmin XI |
| NCBI Official Symbol: | SYT11 |
| NCBI Official Synonym Symbols: | SYT12; sytXI |
| NCBI Protein Information: | synaptotagmin-11; synaptotagmin 12 |
| UniProt Protein Name: | Synaptotagmin-11 |
| UniProt Synonym Protein Names: | Synaptotagmin XI; SytXI |
| Protein Family: | Synaptotagmin |
| UniProt Gene Name: | SYT11 |
| UniProt Entry Name: | SYT11_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SYT11 antibody (CAB8198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human stomach using SYT11 antibody (CAB8198) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using SYT11 antibody (CAB8198) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using SYT11 antibody (CAB8198) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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