Anti-SUV420H1 Antibody (CAB15442)
- SKU:
- CAB15442
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Frequently bought together:
Description
| Antibody Name: | Anti-SUV420H1 Antibody |
| Antibody SKU: | CAB15442 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SUV420H1 (NP_060105.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HeLa, SW480, Mouse uterus, Mouse lung, Rat uterus |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SUV420H1 (NP_060105.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MNTS AFPS RSSR HFSK SDSF SHNN PVRF RPIK GRQE ELKE VIER FKKD EHLE KAFK CLTS GEWA RHYF LNKN KMQE KLFK EHVF IYLR MFAT DSGF EILP C |
| Gene ID: | 51111 |
| Uniprot: | Q4FZB7 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 31kDa/44kDa/99kDa |
| Observed MW: | 110kDa |
| Synonyms: | KMT5B, CGI-85, CGI85, SUV420H1 |
| Background: | This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | SUV420H1: a protein methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Strongly down-regulated in breast cancer cells. Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. Three alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: nucleoplasm; nucleus Molecular Function:histone-lysine N-methyltransferase activity |
| NCBI Summary: | This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q4FZB7 |
| NCBI GenInfo Identifier: | 332278247 |
| NCBI Gene ID: | 51111 |
| NCBI Accession: | Q4FZB7.4 |
| UniProt Secondary Accession: | Q4FZB7,Q3SX56, Q4V775, Q6P150, Q96E44, Q9BUL0, Q9H022 Q9H2K3, Q9NXV3, Q9Y393, B7WNX7, |
| UniProt Related Accession: | Q4FZB7 |
| Molecular Weight: | Calculated: 31kDa/44kDa/99kDaObserved: 92kDa |
| NCBI Full Name: | Histone-lysine N-methyltransferase KMT5B |
| NCBI Synonym Full Names: | lysine methyltransferase 5B |
| NCBI Official Symbol: | KMT5B |
| NCBI Official Synonym Symbols: | CGI85; CGI-85; SUV420H1 |
| NCBI Protein Information: | histone-lysine N-methyltransferase KMT5B |
| UniProt Protein Name: | Histone-lysine N-methyltransferase KMT5B |
| UniProt Synonym Protein Names: | Lysine N-methyltransferase 5B; Lysine-specific methyltransferase 5B |
| Protein Family: | Histone-lysine N-methyltransferase |
| UniProt Gene Name: | KMT5B |
 | Western blot analysis of extracts of various cell lines, using SUV420H1 antibody (CAB15442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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