Anti-STXBP3 Antibody (CAB8153)
- SKU:
- CAB8153
- Product type:
- Antibody
- Application:
- WB
- Application:
- IF
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | STXBP3 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8153 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 343-592 of human STXBP3 (NP_009200.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, U-251MG, Jurkat, Mouse kidney, Mouse lung, Mouse spleen, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 343-592 of human STXBP3 (NP_009200.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KQVV HLNL AEDC MNKF KLNI EKLC KTEQ DLAL GTDA EGQK VKDS MRVL LPVL LNKN HDNC DKIR AILL YIFS INGT TEEN LDRL IQNV KIEN ESDM IRNW SYLG VPIV PQSQ QGKP LRKD RSAE ETFQ LSRW TPFI KDIM EDAI DNRL DSKE WPYC SQCP AVWN GSGA VSAR QKPR ANYL EDRK NGSK LIVF VIGG ITYS EVRC AYEV SQAH KSCE VIIG STHV LTPK KLLD DIKM LNKP KDKV SLIK DE |
| Gene ID: | 6814 |
| Uniprot: | O00186 |
| Cellular Location: | Cell membrane, Cytoplasm, cytosol |
| Calculated MW: | 67kDa |
| Observed MW: | 68kDa |
| Synonyms: | STXBP3, MUNC18-3, MUNC18C, PSP, UNC-18C |
| Background: |
| UniProt Protein Function: | STXBP3: a vesicle protein that, together with STX4 and VAMP2, regulates the insulin-dependent movement of GLUT4 and the docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. Reduction in its expression using siRNA impairs insulin-stimulated GLUT4 vesicle exocytosis in 3T3-L1 adipocytes. Phosphorylated by the insulin receptor (InsR) at Tyr521 in vitro, and bound and phosphorylated by the activated InsR within adipocytes and skeletal muscle. Tyrosine phosphorylation on residues Y219 and Y521 correlates with its dissociation from STX4. Phosphorylated by PKC in platelets in response to thrombin stimulation, inhibiting its binding to STX4. Interacts directly with DOC2B at the cell membrane, excluding its interaction with STX4, regulating glucose-stimulated insulin secretion. Belongs to the STXBP/unc-18/SEC1 family. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 1p13.3 Cellular Component: cytosol; plasma membrane; specific granule Molecular Function:protein binding; syntaxin binding Biological Process: negative regulation of calcium ion-dependent exocytosis; neutrophil degranulation |
| UniProt Code: | O00186 |
| NCBI GenInfo Identifier: | 206729898 |
| NCBI Gene ID: | 6814 |
| NCBI Accession: | O00186.2 |
| UniProt Secondary Accession: | O00186,Q53FW1, Q86YJ3, Q9UPD7, A8K269, A8K5K7, |
| UniProt Related Accession: | O00186 |
| Molecular Weight: | 67,764 Da |
| NCBI Full Name: | Syntaxin-binding protein 3 |
| NCBI Synonym Full Names: | syntaxin binding protein 3 |
| NCBI Official Symbol: | STXBP3 |
| NCBI Official Synonym Symbols: | PSP; MUNC18C; UNC-18C; MUNC18-3 |
| NCBI Protein Information: | syntaxin-binding protein 3 |
| UniProt Protein Name: | Syntaxin-binding protein 3 |
| UniProt Synonym Protein Names: | Platelet Sec1 protein; PSP; Protein unc-18 homolog 3; Unc18-3; Protein unc-18 homolog C; Unc-18C |
| Protein Family: | Syntaxin-binding protein |
| UniProt Gene Name: | STXBP3 |
| UniProt Entry Name: | STXB3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using STXBP3 antibody (CAB8153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using STXBP3 antibody (CAB8153) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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