Anti-STXBP1 Antibody (CAB5420)
- SKU:
- CAB5420
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Zebrafish
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-STXBP1 Antibody |
| Antibody SKU: | CAB5420 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat, Zebrafish |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat, Zebrafish |
| Positive Samples: | HeLa, K-562, Mouse lung, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HIAE VSQE VTRS LKDF SSSK RMNT GEKT TMRD LSQM LKKM PQYQ KELS KYST HLHL AEDC MKHY QGTV DKLC RVEQ DLAM GTDA EGEK IKDP MRAI VPIL LDAN VSTY DKIR IILL YIFL KNGI TEEN LNKL IQHA QIPP EDSE IITN MAHL GVPI VTDS TLRR RSKP ERKE RISE QTYQ LSRW TPII KDIM EDTI EDKL DTKH YPYI STRS SASF STTA VSAR YGHW HKNK APGE YRSG PRLI IFIL GGVS LNEM RCAY EVTQ ANGK WEVL IGST HILT PQKL LDTL KKLN KTDE EISS |
| Gene ID: | 6812 |
| Uniprot: | P61764 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein, cytosol |
| Calculated MW: | 67kDa/68kDa |
| Observed MW: | 68kDa |
| Synonyms: | STXBP1, MUNC18-1, NSEC1, P67, RBSEC1, UNC18 |
| Background: | This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: cytoplasm; cytosol; mitochondrion; myelin sheath; nucleoplasm; plasma membrane; protein complex; terminal button Molecular Function:identical protein binding; protein binding; protein domain specific binding; protein kinase binding; protein N-terminus binding; SNARE binding; syntaxin binding; syntaxin-1 binding Biological Process: axon target recognition; energy reserve metabolic process; glutamate secretion; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; neuromuscular synaptic transmission; neurotransmitter secretion; platelet degranulation; positive regulation of calcium ion-dependent exocytosis; protein stabilization; protein transport; regulation of insulin secretion; regulation of synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle maturation; vesicle docking during exocytosis Disease: Epileptic Encephalopathy, Early Infantile, 4 |
| NCBI Summary: | This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010] |
| UniProt Code: | P61764 |
| NCBI GenInfo Identifier: | 50403646 |
| NCBI Gene ID: | 6812 |
| NCBI Accession: | P61764.1 |
| UniProt Secondary Accession: | P61764,Q28208, Q62759, Q64320, Q96TG8, B1AM97, |
| UniProt Related Accession: | P61764 |
| Molecular Weight: | 68,736 Da |
| NCBI Full Name: | Syntaxin-binding protein 1 |
| NCBI Synonym Full Names: | syntaxin binding protein 1 |
| NCBI Official Symbol: | STXBP1 |
| NCBI Official Synonym Symbols: | P67; NSEC1; UNC18; RBSEC1; MUNC18-1 |
| NCBI Protein Information: | syntaxin-binding protein 1 |
| UniProt Protein Name: | Syntaxin-binding protein 1 |
| UniProt Synonym Protein Names: | MUNC18-1; N-Sec1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A; p67 |
| Protein Family: | Syntaxin-binding protein |
| UniProt Gene Name: | STXBP1 |
| UniProt Entry Name: | STXB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using STXBP1 antibody (CAB5420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat brain using STXBP1 antibody (CAB5420) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spinal cord using STXBP1 antibody (CAB5420) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat brain using STXBP1 antibody (CAB5420) at dilution of 1:50. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse brain using STXBP1 antibody (CAB5420) at dilution of 1:50. Blue: DAPI for nuclear staining. |
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