Anti-STRADA Antibody (CAB18012)[KO Validated]

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SKU:
CAB18012
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-STRADA Antibody [KO Validated]
Antibody SKU:CAB18012
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human STRADA (NP_699166.2).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human STRADA (NP_699166.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FLPE GGCY ELLT VIGK GFED LMTV NLAR YKPT GEYV TVRR INLE ACSN EMVT FLQG ELHV SKLF NHPN IVPY RATF IADN ELWV VTSF MAYG SAKD LICT HFMD GMNE LAIA YILQ GVLK ALDY IHHM GYVH RSVK ASHI LISV DGKV YLSG LRSN LSMI SHGQ RQRV VHDF PKYS VKVL PWLS PE
Gene ID:92335
Uniprot:Q7RTN6
Cellular Location:Cytoplasm, Nucleus
Calculated MW:33kDa/34kDa/38kDa/41kDa/43kDa/48kDa
Observed MW:48kDa
Anti-STRADA Antibody [KO Validated] (CAB18012)Western blot analysis of extracts from normal (control) and STRADA knockout (KO) HeLa cells, using STRADA antibody (CAB18012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Synonyms:STRADA, LYK5, NY-BR-96, PMSE, STRAD, Stlk
Background:The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known.
UniProt Protein Function:STRAD: a pseudokinase which, in complex with CAB39, binds to and activates LKB1. Relocates LKB1 from the nucleus to the cytoplasm. Plays an essential role in LKB1-mediated G1 cell cycle arrest. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; STLK subfamily

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:kinase binding; protein binding; protein kinase activator activity; protein kinase activity; protein serine/threonine kinase activator activity

Biological Process: activation of protein kinase activity; cell cycle arrest; protein amino acid phosphorylation; protein export from nucleus

Disease: Polyhydramnios, Megalencephaly, And Symptomatic Epilepsy

NCBI Summary:The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]
UniProt Code:Q7RTN6
NCBI GenInfo Identifier:74759034
NCBI Gene ID:92335
NCBI Accession:Q7RTN6.1
UniProt Secondary Accession:Q7RTN6,Q5JPI2, Q7Z4K9, Q8NC31, Q8NCF1, Q9H272, B4DDE3 B4DW17, J3KTC9,
UniProt Related Accession:Q7RTN6
Molecular Weight:34,625 Da
NCBI Full Name:STE20-related kinase adapter protein alpha
NCBI Synonym Full Names:STE20-related kinase adaptor alpha
NCBI Official Symbol:STRADA  
NCBI Official Synonym Symbols:LYK5; PMSE; Stlk; STRAD; NY-BR-96  
NCBI Protein Information:STE20-related kinase adapter protein alpha
UniProt Protein Name:STE20-related kinase adapter protein alpha
UniProt Synonym Protein Names:STE20-related adapter protein; Serologically defined breast cancer antigen NY-BR-96
UniProt Gene Name:STRADA  

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