Anti-STK3 Antibody (CAB6992)

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SKU:
CAB6992
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Frequently bought together:

Description

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Antibody Name:Anti-STK3 Antibody
Antibody SKU:CAB6992
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 323-450 of human STK3 (NP_006272.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HeLa, Jurkat, MCF7, NIH/3T3, Mouse liver, Mouse brain, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 323-450 of human STK3 (NP_006272.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SHTM VKTS VESV GTMR ATST MSEG AQTM IEHN STML ESDL GTMV INSE DEEE EDGT MKRN ATSP QVQR PSFM DYFD KQDF KNKS HENC NQNM HEPF PMSK NVFP DNWK VPQD GDFD FLKN LSLE ELQM
Gene ID:6788
Uniprot:Q13188
Cellular Location:Cytoplasm, Nucleus
Calculated MW:56kDa/59kDa
Observed MW:65kDa, 37kDa
Synonyms:KRS1, MST2, STK3
Background:This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.
UniProt Protein Details:

Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Autophagy; EC 2.7.11.1; STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function:protein dimerization activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: central nervous system development; positive regulation of protein binding; protein stabilization; positive regulation of apoptosis; apoptosis; positive regulation of JNK cascade; signal transduction; negative regulation of organ growth; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of fat cell differentiation; positive regulation of transcription factor activity; neural tube formation

NCBI Summary:This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Code:Q13188
NCBI GenInfo Identifier:46577700
NCBI Gene ID:6788
NCBI Accession:Q13188.2
UniProt Secondary Accession:Q13188,Q15445, Q15801, Q96FM6, A8K722, B3KYA7,
UniProt Related Accession:Q13188
Molecular Weight:491
NCBI Full Name:Serine/threonine-protein kinase 3
NCBI Synonym Full Names:serine/threonine kinase 3
NCBI Official Symbol:STK3  
NCBI Official Synonym Symbols:KRS1; MST2  
NCBI Protein Information:serine/threonine-protein kinase 3; MST-2; STE20-like kinase MST2; mammalian STE20-like protein kinase 2; serine/threonine-protein kinase Krs-1; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog)
UniProt Protein Name:Serine/threonine-protein kinase 3
UniProt Synonym Protein Names:Mammalian STE20-like protein kinase 2; MST-2; STE20-like kinase MST2; Serine/threonine-protein kinase Krs-1Cleaved into the following 2 chains:Serine/threonine-protein kinase 3 36kDa subunit; MST2/N; Serine/threonine-protein kinase 3 20kDa subunit; MST2/C
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:STK3  
UniProt Entry Name:STK3_HUMAN
Anti-STK3 Antibody (CAB6992)Western blot analysis of extracts of various cell lines, using STK3 antibody (CAB6992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-STK3 Antibody (CAB6992)Immunohistochemistry of paraffin-embedded human prostate using STK3 antibody (CAB6992) at dilution of 1:100 (40x lens).
Anti-STK3 Antibody (CAB6992)Immunohistochemistry of paraffin-embedded human stomach using STK3 antibody (CAB6992) at dilution of 1:100 (40x lens).
Anti-STK3 Antibody (CAB6992)Immunohistochemistry of paraffin-embedded rat kidney using STK3 antibody (CAB6992) at dilution of 1:100 (40x lens).
Anti-STK3 Antibody (CAB6992)Immunohistochemistry of paraffin-embedded mouse stomach using STK3 antibody (CAB6992) at dilution of 1:100 (40x lens).

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