Anti-STK24 항체 (CAB10576)

Antibody Name:	Anti-STK24 Antibody
Antibody SKU:	CAB10576
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 312-431 of human STK24 (NP_001027467.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A375, U-251MG, HT-29, NIH/3T3, Mouse liver, Mouse kidney, Mouse lung, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 312-431 of human STK24 (NP_001027467.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TDGQ ASGG SDSG DWIF TIRE KDPK NLEN GALQ PSDL DRNK MKDI PKRP FSQC LSTI ISPL FAEL KEKS QACG GNLG SIEE LRGA IYLA EEAC PGIS DTMV AQLV QRLQ RYSL SGGG TSSH
Gene ID:	8428
Uniprot:	Q9Y6E0
Cellular Location:	Cytoplasm, Membrane, Nucleus
Calculated MW:	47kDa/49kDa
Observed MW:	49kDa

Synonyms:

STK24, HEL-S-95, MST3, MST3B, STE20, STK3

Background:

This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MST3: a protein kinase of the STE20 family. Activated by caspase-mediated cleavage
UniProt Protein Details:	Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; STE group; STE20 family; YSK subfamily Chromosomal Location of Human Ortholog: 13q31.2-q32.3 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity Biological Process: regulation of axon regeneration; response to hydrogen peroxide; apoptosis; protein amino acid autophosphorylation; signal transduction; induction of apoptosis by oxidative stress; protein amino acid phosphorylation; negative regulation of cell migration; cell structure disassembly during apoptosis
NCBI Summary:	This gene encodes a serine/threonine protein kinase that functions upstream of mitogen-activated protein kinase (MAPK) signaling. The encoded protein is cleaved into two chains by caspases; the N-terminal fragment (MST3/N) translocates to the nucleus and promotes programmed cells death. There is a pseudogene for this gene on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
UniProt Code:	Q9Y6E0
NCBI GenInfo Identifier:	13626607
NCBI Gene ID:	8428
NCBI Accession:	Q9Y6E0.1
UniProt Secondary Accession:	Q9Y6E0,O14840, Q5JV92,
UniProt Related Accession:	Q9Y6E0
Molecular Weight:	47,913 Da
NCBI Full Name:	Serine/threonine-protein kinase 24
NCBI Synonym Full Names:	serine/threonine kinase 24
NCBI Official Symbol:	STK24
NCBI Official Synonym Symbols:	MST3; STK3; MST3B; STE20; HEL-S-95
NCBI Protein Information:	serine/threonine-protein kinase 24; STE20-like kinase 3; STE20-like kinase MST3; sterile 20-like kinase 3; epididymis secretory protein Li 95; mammalian STE20-like protein kinase 3; serine/threonine kinase 24 (STE20 homolog, yeast)
UniProt Protein Name:	Serine/threonine-protein kinase 24
UniProt Synonym Protein Names:	Mammalian STE20-like protein kinase 3; MST-3; STE20-like kinase MST3Cleaved into the following 2 chains:Serine/threonine-protein kinase 24 36 kDa subunitAlternative name(s):Mammalian STE20-like protein kinase 3 N-terminal; MST3/N
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	STK24
UniProt Entry Name:	STK24_HUMAN

	Western blot analysis of extracts of various cell lines, using STK24 antibody (CAB10576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat lung using STK24 antibody (CAB10576) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using STK24 antibody (CAB10576) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using STK24 antibody (CAB10576) at dilution of 1:100 (40x lens).

Antibodies

STK24 Antibody (PACO01115)

STK24 Antibody (PACO12530)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)