Anti-STING / TMEM173 Antibody (CAB3575)
- SKU:
- CAB3575
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-STING / TMEM173 Antibody |
| Antibody SKU: | CAB3575 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-379 of human STING / TMEM173 (NP_938023.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, THP-1, Mouse spleen, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-379 of human STING / TMEM173 (NP_938023.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LILP ELQA RIRT YNQH YNNL LRGA VSQR LYIL LPLD CGVP DNLS MADP NIRF LDKL PQQT GDHA GIKD RVYS NSIY ELLE NGQR AGTC VLEY ATPL QTLF AMSQ YSQA GFSR EDRL EQAK LFCR TLED ILAD APES QNNC RLIA YQEP ADDS SFSL SQEV LRHL RQEE KEEV TVGS LKTS AVPS TSTM SQEP ELLI SGME KPLP LRTD FS |
| Gene ID: | 340061 |
| Uniprot: | Q86WV6 |
| Cellular Location: | Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion outer membrane, Multi-pass membrane protein, perinuclear region |
| Calculated MW: | 42kDa |
| Observed MW: | 39kDa |
| Synonyms: | TMEM173, ERIS, MITA, MPYS, NET23, SAVI, STING, hMITA, hSTING |
| Background: | This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | TMEM173: Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double- stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti- viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Associates with the MHC-II complex. Homodimer; 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization. Interacts with DDX58/RIG-I, MAVS/VISA and SSR2. Interacts with RNF5 and TRIM56. Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta. Ubiquitously expressed. Belongs to the TMEM173 family. |
| UniProt Protein Details: | Protein type:Mitochondrial; Membrane protein, multi-pass; Apoptosis; Membrane protein, integral; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 5q31.2 Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; mitochondrial outer membrane; cytoplasmic vesicle membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; peroxisome Molecular Function:identical protein binding; protein binding; protein homodimerization activity; ubiquitin protein ligase binding; transcription factor binding; protein kinase binding Biological Process: regulation of interferon type I production; positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; apoptosis; positive regulation of interferon type I production; positive regulation of transcription factor import into nucleus; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; activation of innate immune response; defense response to virus; interferon-beta production Disease: Sting-associated Vasculopathy, Infantile-onset |
| NCBI Summary: | This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
| UniProt Code: | Q86WV6 |
| NCBI GenInfo Identifier: | 74727720 |
| NCBI Gene ID: | 340061 |
| NCBI Accession: | Q86WV6.1 |
| UniProt Secondary Accession: | Q86WV6,A8K3P6, B6EB35, D6RBX0, D6RE01, D6RID9, |
| UniProt Related Accession: | Q86WV6 |
| Molecular Weight: | Observed MW: 39kDa; Calculated MW 42kDa |
| NCBI Full Name: | Stimulator of interferon genes protein |
| NCBI Synonym Full Names: | transmembrane protein 173 |
| NCBI Official Symbol: | TMEM173 |
| NCBI Official Synonym Symbols: | ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING |
| NCBI Protein Information: | stimulator of interferon genes protein; endoplasmic reticulum IFN stimulator; mitochondrial mediator of IRF3 activation; endoplasmic reticulum interferon stimulator; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner |
| UniProt Protein Name: | Stimulator of interferon genes protein |
| UniProt Synonym Protein Names: | Endoplasmic reticulum interferon stimulator; ERIS; Mediator of IRF3 activation; hMITA; Transmembrane protein 173 |
| Protein Family: | Stimulator of interferon genes protein |
| UniProt Gene Name: | TMEM173 |
| UniProt Entry Name: | STING_HUMAN |
 | Western blot analysis of extracts of various cell lines, using STING / TMEM173 antibody (CAB3575) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat pancreas using STING / TMEM173 antibody (CAB3575) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using STING / TMEM173 antibody (CAB3575) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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