Anti-ST8SIA2 항체 (CAB7748)

Antibody Name:	Anti-ST8SIA2 Antibody
Antibody SKU:	CAB7748
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human ST8SIA2 (NP_006002.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, SKOV3, Mouse testis, Mouse brain, Mouse heart, Mouse lung, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human ST8SIA2 (NP_006002.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DISE IEEE IGNS GGRG TIRS AVNS LHSK SNRA EVVI NGSS SPAV VDRS NESI KHNI QPAS SKWR HNQT LSLR IRKQ ILKF LDAE KDIS VLKG TLKP GDII HYIF DRDS TMNV SQNL YELL PRTS PLKN KHFG TCAI V
Gene ID:	8128
Uniprot:	Q92186
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	42kDa
Observed MW:	42kDa

Synonyms:

ST8SIA2, HsT19690, SIAT8-B, SIAT8B, ST8SIA-II, ST8SiaII, STX, alpha-2

Background:

The protein encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29.

UniProt Protein Function:	ST8SIA2: May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression. Belongs to the glycosyltransferase 29 family.
UniProt Protein Details:	Protein type:EC 2.4.99.-; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; Membrane protein, integral; Transferase Chromosomal Location of Human Ortholog: 15q26 Cellular Component: Golgi membrane; recycling endosome; early endosome; integral to Golgi membrane Molecular Function:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity; sialic acid binding Biological Process: axon guidance; nervous system development; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate metabolic process; protein modification process; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; N-glycan processing; post-translational protein modification; oligosaccharide metabolic process; ganglioside biosynthetic process
NCBI Summary:	The protein encoded by this gene is a type II membrane protein that is thought to catalyze the transfer of sialic acid from CMP-sialic acid to N-linked oligosaccharides and glycoproteins. The encoded protein may be found in the Golgi apparatus and may be involved in the production of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). This protein is a member of glycosyltransferase family 29. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92186
NCBI GenInfo Identifier:	2494831
NCBI Gene ID:	8128
NCBI Accession:	Q92186.1
UniProt Secondary Accession:	Q92186,Q4VAZ0, Q92470, Q92746,
UniProt Related Accession:	Q92186
Molecular Weight:	42,430 Da
NCBI Full Name:	Alpha-2,8-sialyltransferase 8B
NCBI Synonym Full Names:	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
NCBI Official Symbol:	ST8SIA2
NCBI Official Synonym Symbols:	STX; SIAT8B; HsT19690; ST8SIA-II
NCBI Protein Information:	alpha-2,8-sialyltransferase 8B; SIAT8-B; ST8SiaII; sialyltransferase X; sialytransferase St8Sia II; sialyltransferase St8Sia II; alpha-2,8-sialyltransferase 8B 1; sialyltransferase 8 (alpha-2, 8-sialytransferase) B
UniProt Protein Name:	Alpha-2,8-sialyltransferase 8B
UniProt Synonym Protein Names:	Sialyltransferase 8B; SIAT8-B; Sialyltransferase St8Sia II; ST8SiaII; Sialyltransferase X; STX
Protein Family:	Alpha-2,8-sialyltransferase
UniProt Gene Name:	ST8SIA2
UniProt Entry Name:	SIA8B_HUMAN

	Western blot analysis of extracts of various cell lines, using ST8SIA2 antibody (CAB7748) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using ST8SIA2 antibody (CAB7748) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using ST8SIA2 antibody (CAB7748) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using ST8SIA2 antibody (CAB7748) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)