Anti-SRI Antibody (CAB19921)[KO Validated]

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SKU:
CAB19921
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-SRI Antibody [KO Validated]
Antibody SKU:CAB19921
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human SRI.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant protein of human SRI.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6717
Uniprot:P30626
Cellular Location:Cytoplasm, Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane
Calculated MW:20kDa/21kDa
Observed MW:18KDa
Anti-SRI Antibody [KO Validated] (CAB19921)Western blot analysis of extracts from normal (control) and SRI knockout (KO) HeLa cells, using SRI antibody (CAB19921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:SRI, CP-22, CP22, SCN, V19, sorcin
Background:This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene.
UniProt Protein Function:SRI: Calcium-binding protein that modulates excitation- contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.
UniProt Protein Details:

Protein type:Calcium-binding

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; membrane; mitochondrion; sarcoplasmic reticulum; T-tubule; cytoplasm; nerve terminal; cytosol; Z disc

Molecular Function:protein binding; protease binding; protein heterodimerization activity; calcium channel regulator activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: regulation of action potential; cytoplasmic sequestering of transcription factor; muscle development; negative regulation of heart rate; heart development; regulation of striated muscle contraction; regulation of calcium ion transport; proteolysis; signal transduction; regulation of heart contraction; transport; calcium ion transport; positive regulation of release of sequestered calcium ion into cytosol; intracellular sequestering of iron ion

NCBI Summary:This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]
UniProt Code:P30626
NCBI GenInfo Identifier:267021
NCBI Gene ID:6717
NCBI Accession:P30626.1
UniProt Secondary Accession:P30626,A8MTH6, B4DKK2, D6W5Q0,
UniProt Related Accession:P30626
Molecular Weight:198
NCBI Full Name:Sorcin
NCBI Synonym Full Names:sorcin
NCBI Official Symbol:SRI  
NCBI Official Synonym Symbols:SCN; V19; CP22; CP-22  
NCBI Protein Information:sorcin; H_RG167B05.1; 22 kDa protein; calcium binding protein amplified in mutlidrug-resistant cells
UniProt Protein Name:Sorcin
UniProt Synonym Protein Names:22 kDa protein; CP-22; CP22; V19
UniProt Gene Name:SRI  
UniProt Entry Name:SORCN_HUMAN

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