Anti-SRGN Antibody (CAB6951)
- SKU:
- CAB6951
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-SRGN Antibody |
| Antibody SKU: | CAB6951 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-158 of human SRGN (NP_002718.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human |
| Positive Samples: | THP-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-158 of human SRGN (NP_002718.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YPTR RARY QWVR CNPD SNSA NCLE EKGP MFEL LPGE SNKI PRLR TDLF PKTR IQDL NRIF PLSE DYSG SGFG SGSG SGSG SGSG FLTE MEQD YQLV DESD AFHD NLRS LDRN LPSD SQDL GQHG LEED FML |
| Gene ID: | 5552 |
| Uniprot: | P10124 |
| Cellular Location: | Cytoplasmic granule, Golgi apparatus, Secreted, extracellular space |
| Calculated MW: | 17kDa |
| Observed MW: | 17kDa |
| Synonyms: | SRGN, PPG, PRG, PRG1, serglycin |
| Background: | This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. |
| UniProt Protein Function: | SRGN: Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF- alpha and may also regulate protease secretion. Inhibits bone mineralization. Belongs to the serglycin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; mast cell granule; extracellular region Molecular Function:collagen binding; protein binding Biological Process: maintenance of granzyme B localization in T cell secretory granule; platelet activation; negative regulation of bone mineralization; platelet degranulation; induction of apoptosis by granzyme; biomineral formation; negative regulation of cytokine secretion; T cell secretory granule organization and biogenesis; maintenance of protease localization in mast cell secretory granule; protein processing; mast cell secretory granule organization and biogenesis; blood coagulation |
| NCBI Summary: | This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P10124 |
| NCBI GenInfo Identifier: | 269849659 |
| NCBI Gene ID: | 5552 |
| NCBI Accession: | P10124.3 |
| UniProt Secondary Accession: | P10124,Q5VW06, B2R4L7, |
| UniProt Related Accession: | P10124 |
| Molecular Weight: | 158 |
| NCBI Full Name: | Serglycin |
| NCBI Synonym Full Names: | serglycin |
| NCBI Official Symbol: | SRGN |
| NCBI Official Synonym Symbols: | PPG; PRG; PRG1 |
| NCBI Protein Information: | serglycin; p.PG; serglycin proteoglycan; proteoglycan 1, secretory granule; platelet proteoglycan core protein; hematopoetic proteoglycan core peptide; hematopoetic proteoglycan core protein; hematopoietic proteoglycan core protein; secretory granule proteoglycan core peptide; secretory granule proteoglycan core protein; proteoglycan protein core for mast cell secretory granule |
| UniProt Protein Name: | Serglycin |
| UniProt Synonym Protein Names: | Hematopoietic proteoglycan core protein; Platelet proteoglycan core protein; P.PG; Secretory granule proteoglycan core protein |
| Protein Family: | Serglycin |
| UniProt Gene Name: | SRGN |
| UniProt Entry Name: | SRGN_HUMAN |
 | Western blot analysis of extracts of THP-1 cells, using SRGN antibody (CAB6951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using SRGN antibody (CAB6951) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using SRGN antibody (CAB6951). Blue: DAPI for nuclear staining. |
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