Anti-SREBP1c Antibody (CAB15586)
- SKU:
- CAB15586
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-SREBP1c Antibody |
| Antibody SKU: | CAB15586 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SREBF1 (NP_001005291.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Mouse kidney, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SREBF1 (NP_001005291.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDEP PFSE AALE QALG EPCD LDAA LLTD IEGE VGAG RGRA NGLD APRA GADR GAMD CTFE DMLQ LINN QDSD FPGL FDPP YAGS GAGG TDPA SPDT SSPG |
| Gene ID: | 6720 |
| Uniprot: | P36956 |
| Cellular Location: | COPII-coated vesicle membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, Multi-pass membrane protein, Nucleus |
| Calculated MW: | 45kDa/48kDa/111kDa/113kDa/121kDa/124kDa |
| Observed MW: | 70kDa |
| Synonyms: | SREBF1, SREBP-1c, SREBP1, SREBP1a, bHLHd1 |
| Background: | This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. |
| UniProt Protein Function: | Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). |
| NCBI Summary: | This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016] |
| UniProt Code: | P36956 |
| NCBI GenInfo Identifier: | 166897633 |
| NCBI Gene ID: | 6720 |
| NCBI Accession: | P36956.2 |
| UniProt Secondary Accession: | P36956,Q16062, Q59F52, Q6P4R7, Q6PFW7, Q6PJ36, Q8TAK9 B0I4X3, B0I4X4, D3DXC4, |
| UniProt Related Accession: | P36956 |
| Molecular Weight: | 45,816 Da |
| NCBI Full Name: | Sterol regulatory element-binding protein 1 |
| NCBI Synonym Full Names: | sterol regulatory element binding transcription factor 1 |
| NCBI Official Symbol: | SREBF1 |
| NCBI Official Synonym Symbols: | SREBP1; bHLHd1; SREBP1a; SREBP-1c |
| NCBI Protein Information: | sterol regulatory element-binding protein 1 |
| UniProt Protein Name: | Sterol regulatory element-binding protein 1 |
| UniProt Synonym Protein Names: | Class D basic helix-loop-helix protein 1; bHLHd1 |
| UniProt Gene Name: | SREBF1 |
| UniProt Entry Name: | SRBP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SREBP1c antibody (CAB15586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
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