Anti-SPTA1 Antibody (CAB12355)
- SKU:
- CAB12355
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SPTA1 Antibody |
| Antibody SKU: | CAB12355 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human SPTA1 (NP_003117.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 239T, LO2, A-549, Mouse kidney, Mouse liver, Mouse lung, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human SPTA1 (NP_003117.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KHEA FLLD LNSF GDSM KALR NQAN ACQQ QQAA PVEG VAGE QRVM ALYD FQAR SPRE VTMK KGDV LTLL SSIN KDWW KVEA ADHQ GIVP AVYV RRLA HDEF PMLP QRRR EEPG NITQ RQEQ IENQ YRSL LDRA EERR RRLL QRYN EFLL AYEA GDML EWIQ EKKA ENTG VELD DVWE LQKK FDEF QKDL NTNE PRLR DINK VADD LLFE GLLT PEGA QIRQ E |
| Gene ID: | 6708 |
| Uniprot: | P02549 |
| Cellular Location: | Cytoplasm, cell cortex, cytoskeleton |
| Calculated MW: | 279kDa/280kDa |
| Observed MW: | 300kDa |
| Synonyms: | SPTA1, EL2, HPP, HS3, SPH3, SPTA |
| Background: | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. |
| UniProt Protein Function: | SPTA1: Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Defects in SPTA1 are the cause of elliptocytosis type 2 (EL2). EL2 is a Rhesus-unlinked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape. Defects in SPTA1 are a cause of hereditary pyropoikilocytosis (HPP). HPP is an autosomal recessive disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells. Defects in SPTA1 are the cause of spherocytosis type 3 (SPH3); also known as hereditary spherocytosis type 3 (HS3). Spherocytosis is a hematologic disorder leading to chronic hemolytic anemia and characterized by numerous abnormally shaped erythrocytes which are generally spheroidal. SPH3 is characterized by severe hemolytic anemia. Inheritance is autosomal recessive. Belongs to the spectrin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q21 Cellular Component: spectrin; cytosol; actin cytoskeleton Molecular Function:actin filament binding; protein binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding Biological Process: regulation of cell shape; axon guidance; positive regulation of protein binding; plasma membrane organization and biogenesis; actin filament capping; actin filament organization; positive regulation of T cell proliferation; hemopoiesis; lymphocyte homeostasis; porphyrin biosynthetic process Disease: Elliptocytosis 2; Pyropoikilocytosis, Hereditary; Spherocytosis, Type 3 |
| NCBI Summary: | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P02549 |
| NCBI GenInfo Identifier: | 308153675 |
| NCBI Gene ID: | 6708 |
| NCBI Accession: | P02549.5 |
| UniProt Secondary Accession: | P02549,Q15514, Q5VYL1, Q5VYL2, Q6LDY5, |
| UniProt Related Accession: | P02549 |
| Molecular Weight: | 279,674 Da |
| NCBI Full Name: | Spectrin alpha chain, erythrocytic 1 |
| NCBI Synonym Full Names: | spectrin, alpha, erythrocytic 1 |
| NCBI Official Symbol: | SPTA1 |
| NCBI Official Synonym Symbols: | EL2; HPP; HS3; SPH3; SPTA |
| NCBI Protein Information: | spectrin alpha chain, erythrocytic 1; alpha-I spectrin; elliptocytosis 2; erythroid alpha-spectrin; spectrin alpha chain, erythrocyte; spectrin, alpha, erythrocytic 1 (elliptocytosis 2) |
| UniProt Protein Name: | Spectrin alpha chain, erythrocytic 1 |
| UniProt Synonym Protein Names: | Erythroid alpha-spectrin |
| Protein Family: | Spectrin |
| UniProt Gene Name: | SPTA1 |
| UniProt Entry Name: | SPTA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPTA1 antibody (CAB12355) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human tonsil using SPTA1 antibody (CAB12355) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat spleen using SPTA1 antibody (CAB12355) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using SPTA1 antibody (CAB12355) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat bone marrow cells using SPTA1 antibody (CAB12355) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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