Anti-SPIN1 Antibody (CAB5853)
- SKU:
- CAB5853
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-SPIN1 Antibody |
| Antibody SKU: | CAB5853 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIN1 (NP_006708.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SW480, 293T, Jurkat, HeLa, HepG2, SH-SY5Y, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIN1 (NP_006708.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKTP FGKT PGQR SRAD AGHA GVSA NMMK KRTS HKKH RSSV GPSK PVSQ PRRN IVGC RIQH GWKE GNGP VTQW KGTV LDQV PVNP SLYL IKYD GFDC VYGL ELNK DERV SALE VLPD RVAT SRIS DAHL ADTM IGKA VEHM FETE DGSK DEWR GMVL ARAP VMNT WFYI TYEK DPVL YMYQ LLDD YKEG DLRI MPDS NDSP PAER EPGE VVDS LVGK QVEY AKED GSKR TGMV IHQV EAKP SVYF IKFD DDFH IYVY DLVK TS |
| Gene ID: | 10927 |
| Uniprot: | Q9Y657 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 29kDa |
| Observed MW: | 32kDa |
| Synonyms: | SPIN1, SPIN, TDRD24 |
| Background: |
| UniProt Protein Function: | SPIN1: May play a role in cell-cycle regulation during the transition from gamete to embryo. Belongs to the SPIN/STSY family. |
| UniProt Protein Details: | Protein type:Nucleolus; DNA-binding Chromosomal Location of Human Ortholog: 9q22.1 Cellular Component: nucleolus; spindle; nucleus Molecular Function:protein binding; methylated histone residue binding Biological Process: Wnt receptor signaling pathway; meiotic cell cycle; multicellular organismal development; gamete generation; chromatin modification; positive regulation of Wnt receptor signaling pathway; rRNA transcription |
| UniProt Code: | Q9Y657 |
| NCBI GenInfo Identifier: | 93141317 |
| NCBI Gene ID: | 10927 |
| NCBI Accession: | Q9Y657.3 |
| UniProt Secondary Accession: | Q9Y657,Q7KZJ8, Q9GZT2, Q9H0N7, A8K0X6, B3KRQ4, |
| UniProt Related Accession: | Q9Y657 |
| Molecular Weight: | Calculated MW: 29kDaObserved MW: 32kDa |
| NCBI Full Name: | Spindlin-1 |
| NCBI Synonym Full Names: | spindlin 1 |
| NCBI Official Symbol: | SPIN1 |
| NCBI Official Synonym Symbols: | SPIN |
| NCBI Protein Information: | spindlin-1; ovarian cancer-related protein; spindlin1 |
| UniProt Protein Name: | Spindlin-1 |
| UniProt Synonym Protein Names: | Ovarian cancer-related protein; Spindlin1 |
| Protein Family: | Spindlin |
| UniProt Gene Name: | SPIN1 |
| UniProt Entry Name: | SPIN1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SPIN1 antibody (CAB5853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using SPIN1 antibody (CAB5853) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using SPIN1 antibody (CAB5853) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using SPIN1 antibody (CAB5853). Blue: DAPI for nuclear staining. |
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