Anti-SPICE1 Antibody (CAB7855)
- SKU:
- CAB7855
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-SPICE1 Antibody |
| Antibody SKU: | CAB7855 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SPICE1 (NP_653319.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HeLa, BT-474, MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SPICE1 (NP_653319.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSFV RVNR CGPR VGVR KTPK VKKK KTSV KQEW DNTV TDLT VHRA TPED LVRR HEIH KSKN RALV HWEL QEKA LKRK WRKQ KPET LNLE KRRL SIMK EILS DQYQ MQDV LEKS DHLI AAAK ELFP RRRT GFPN VTVA PDSS QGPI VVNQ DPIT QSIF NESV IEPQ ALND VDGE EEGT VNSQ SGES ENEN ELDN SLNS QSNT NTDR FLQQ LTEE NFEL ISKL |
| Gene ID: | 152185 |
| Uniprot: | Q8N0Z3 |
| Cellular Location: | Cytoplasm, centriole, centrosome, cytoskeleton, microtubule organizing center, spindle |
| Calculated MW: | 96kDa |
| Observed MW: | 120kDa |
| Synonyms: | SPICE1, CCDC52, SPICE |
| Background: |
| UniProt Protein Function: | CCDC52: a protein of unknown function. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 3q13.2 Cellular Component: centriole; centrosome; spindle Molecular Function:protein binding Biological Process: metaphase plate congression; regulation of centriole replication |
| UniProt Code: | Q8N0Z3 |
| NCBI GenInfo Identifier: | 74728478 |
| NCBI Gene ID: | 152185 |
| NCBI Accession: | Q8N0Z3.1 |
| UniProt Secondary Accession: | Q8N0Z3,Q8WUX6, D3DN72, |
| UniProt Related Accession: | Q8N0Z3 |
| Molecular Weight: | 96kDa |
| NCBI Full Name: | Spindle and centriole-associated protein 1 |
| NCBI Synonym Full Names: | spindle and centriole associated protein 1 |
| NCBI Official Symbol: | SPICE1 |
| NCBI Official Synonym Symbols: | SPICE; CCDC52 |
| NCBI Protein Information: | spindle and centriole-associated protein 1 |
| UniProt Protein Name: | Spindle and centriole-associated protein 1 |
| UniProt Synonym Protein Names: | Coiled-coil domain-containing protein 52; Spindle and centriole-associated protein |
| Protein Family: | Spindle and centriole-associated protein |
| UniProt Gene Name: | SPICE1 |
 | Western blot analysis of extracts of various cell lines, using SPICE1 antibody (CAB7855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using SPICE1 antibody (CAB7855) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using SPICE1 antibody (CAB7855) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using SPICE1 antibody (CAB7855) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using SPICE1 antibody (CAB7855) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Related Products
Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit
Human Anti-DSG3 antibody(anti-Desmoglein-3 antibody) ELISA Kit
Anti-SLC20A2 Antibody (CAB6739)
