Anti-Sorting nexin-1 Antibody (CAB8625)
- SKU:
- CAB8625
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Sorting nexin-1 Antibody |
| Antibody SKU: | CAB8625 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNX1 (NP_003090.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:1000 - 1:2000 IF 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, LO2, A-549, HT-1080 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SNX1 (NP_003090.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASG GGGC SASE RLPP PFPG LEPE SEGA AGGS EPEA GDSD TEGE DIFT GAAV VSKH QSPK ITTS LLPI NNGS KENG IHEE QDQE PQDL FADA TVEL SLDS TQNN QKKV LAKT LISL PPQE ATNS SKPQ PTYE ELEE EEQE DQFD LTVG IT |
| Gene ID: | 6642 |
| Uniprot: | Q13596 |
| Cellular Location: | Cell projection, Cytoplasmic side, Early endosome membrane, Endosome membrane, Golgi apparatus, Peripheral membrane protein, lamellipodium, trans-Golgi network membrane |
| Calculated MW: | 51kDa/59kDa/63kDa |
| Observed MW: | 80kDa |
| Synonyms: | SNX1, HsT17379, VPS5 |
| Background: | This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | SNX1: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3- phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Belongs to the sorting nexin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Vesicle Chromosomal Location of Human Ortholog: 15q22.31 Cellular Component: cytoplasm; early endosome membrane; endosome membrane; extrinsic to membrane; intracellular membrane-bound organelle; membrane; protein complex; retromer complex Molecular Function:epidermal growth factor receptor binding; identical protein binding; insulin receptor binding; phosphoinositide binding; protein binding; protein heterodimerization activity; protein homodimerization activity Biological Process: intracellular protein transport; receptor internalization; retrograde transport, endosome to Golgi; vesicle organization and biogenesis |
| NCBI Summary: | This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13596 |
| NCBI GenInfo Identifier: | 17380569 |
| NCBI Gene ID: | 6642 |
| NCBI Accession: | Q13596.3 |
| UniProt Secondary Accession: | Q13596,O60750, O60751, Q6ZRJ8, A6NM19, A8K6T7, H0Y2M5 |
| UniProt Related Accession: | Q13596 |
| Molecular Weight: | 63,082 Da |
| NCBI Full Name: | Sorting nexin-1 |
| NCBI Synonym Full Names: | sorting nexin 1 |
| NCBI Official Symbol: | SNX1 |
| NCBI Official Synonym Symbols: | VPS5; HsT17379 |
| NCBI Protein Information: | sorting nexin-1 |
| UniProt Protein Name: | Sorting nexin-1 |
| Protein Family: | Sorting nexin |
| UniProt Gene Name: | SNX1 |
| UniProt Entry Name: | SNX1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SNX1 antibody (CAB8625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of L929 cells using SNX1 Rabbit pAb (CAB8625) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using SNX1 Rabbit pAb (CAB8625) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining. |
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