Anti-SOCS3 Antibody (CAB0694)
- SKU:
- CAB0694
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SOCS3 Antibody |
| Antibody SKU: | CAB0694 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human SOCS3 (NP_003946.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse spleen |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human SOCS3 (NP_003946.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GSFS LQSD PRST QPVP RFDC VLKL VHHY MPPP GAPS FPSP PTEP SSEV PEQP SAQP LPGS PPRR AYYI YSGG EKIP LVLS RPLS SNVA TLQH LCRK TVNG HLDS YEKV TQLP GPIR EFLD QYDA PL |
| Gene ID: | 9021 |
| Uniprot: | O14543 |
| Cellular Location: | |
| Calculated MW: | 24kDa |
| Observed MW: | 30kDa |
| Synonyms: | SOCS3, ATOD4, CIS3, Cish3, SOCS-3, SSI-3, SSI3 |
| Background: | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. |
| UniProt Protein Function: | SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 17q25.3 Cellular Component: cytoplasm; cytosol Molecular Function:protein binding; protein kinase inhibitor activity Biological Process: regulation of growth; negative regulation of inflammatory response; cytokine and chemokine mediated signaling pathway; protein ubiquitination; negative regulation of protein kinase activity; negative regulation of insulin receptor signaling pathway; JAK-STAT cascade; positive regulation of cell differentiation; negative regulation of JAK-STAT cascade; negative regulation of apoptosis Disease: Dermatitis, Atopic, 4 |
| NCBI Summary: | This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14543 |
| NCBI GenInfo Identifier: | 20178094 |
| NCBI Gene ID: | 9021 |
| NCBI Accession: | O14543.1 |
| UniProt Secondary Accession: | O14543,O14509, |
| UniProt Related Accession: | O14543 |
| Molecular Weight: | |
| NCBI Full Name: | Suppressor of cytokine signaling 3 |
| NCBI Synonym Full Names: | suppressor of cytokine signaling 3 |
| NCBI Official Symbol: | SOCS3 |
| NCBI Official Synonym Symbols: | CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3 |
| NCBI Protein Information: | suppressor of cytokine signaling 3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3 |
| UniProt Protein Name: | Suppressor of cytokine signaling 3 |
| UniProt Synonym Protein Names: | Cytokine-inducible SH2 protein 3; CIS-3; STAT-induced STAT inhibitor 3; SSI-3 |
| Protein Family: | Suppressor of cytokine signaling |
| UniProt Gene Name: | SOCS3 |
| UniProt Entry Name: | SOCS3_HUMAN |
 | Western blot analysis of extracts of mouse spleen, using SOCS3 antibody (CAB0694) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse heart using SOCS3 Rabbit pAb (CAB0694) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using SOCS3 Rabbit pAb (CAB0694) at dilution of 1:50 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using SOCS3 Rabbit pAb (CAB0694) at dilution of 1:50 (40x lens). |
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