Anti-SNX15 항체 (CAB9158)

Antibody Name:	Anti-SNX15 Antibody
Antibody SKU:	CAB9158
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human SNX15 (NP_037438.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HepG2, Jurkat, Raji, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-342 of human SNX15 (NP_037438.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSRQ AKDD FLRH YTVS DPRT HPKG YTEY KVTA QFIS KKDP EDVK EVVV WKRY SDFR KLHG DLAY THRN LFRR LEEF PAFP RAQV FGRF EASV IEER RKGA EDLL RFTV HIPA LNNS PQLK EFFR GGEV TRPL EVSR DLHI LPPP LIPT PPPD DPRL SQLL PAER RGLE ELEV PVDP PPSS PAQE ALDL LFNC ESTE EASG SPAR GPLT EAEL ALFD PFSK EEGA APSP THVA ELAT MEVE SARL DQEP WEPG GQEE EEDG EGGP TPAY LSQA TELI TQAL RDEK AGAY AAAL QGYR DGVH VLLQ GVPS DPLP ARQE GVKK KAAE YLKR AEEI LRLH LSQL PP
Gene ID:	29907
Uniprot:	Q9NRS6
Cellular Location:	Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle membrane, Membrane, Peripheral membrane protein
Calculated MW:	29kDa/38kDa
Observed MW:	50kDa

Synonyms:

SNX15, HSAF001435

Background:

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits. This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in endosomal trafficking from the plasma membrane to recycling endosomes or the trans-Golgi network. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ADP-ribosylation factor-like 2 (ARL2) gene.

UniProt Protein Function:	SNX15: May be involved in several stages of intracellular trafficking. Overexpression of SNX15 disrupts the normal trafficking of proteins from the plasma membrane to recycling endosomes or the TGN. Belongs to the sorting nexin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 11q12 Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleolus Molecular Function:protein binding; protein serine/threonine kinase activity Biological Process: signal transduction
NCBI Summary:	This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits. This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in endosomal trafficking from the plasma membrane to recycling endosomes or the trans-Golgi network. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ADP-ribosylation factor-like 2 (ARL2) gene. [provided by RefSeq, Dec 2010]
UniProt Code:	Q9NRS6
NCBI GenInfo Identifier:	13124562
NCBI Gene ID:	29907
NCBI Accession:	Q9NRS6.1
UniProt Secondary Accession:	Q9NRS6,Q9NRS5, E5KQS6,
UniProt Related Accession:	Q9NRS6
Molecular Weight:	29,220 Da
NCBI Full Name:	Sorting nexin-15
NCBI Synonym Full Names:	sorting nexin 15
NCBI Official Symbol:	SNX15
NCBI Official Synonym Symbols:	HSAF001435
NCBI Protein Information:	sorting nexin-15
UniProt Protein Name:	Sorting nexin-15
Protein Family:	Sorting nexin
UniProt Gene Name:	SNX15
UniProt Entry Name:	SNX15_HUMAN

	Western blot analysis of extracts of various cell lines, using SNX15 antibody (CAB9158) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using SNX15 antibody (CAB9158) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using SNX15 antibody (CAB9158) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SNX15 antibody (CAB9158) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

SNX15 Antibody (PACO12377)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)