Anti-SNRNP25 Antibody (CAB15511)

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SKU:
CAB15511
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-SNRNP25 Antibody
Antibody SKU:CAB15511
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human SNRNP25 (NP_078847.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, A-549, K-562, MCF7, rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human SNRNP25 (NP_078847.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDVF QEGL AMVV QDPL LCDL PIQV TLEE VNSQ IALE YGQA MTVR VCKM DGEV MPVV VVQS ATVL DLKK AIQR YVQL KQER EGGI QHIS WSYV WRTY HLTS AGEK LTED RKKL RDYG IRNR DEVS FIKK LRQK
Gene ID:79622
Uniprot:Q9BV90
Cellular Location:Nucleus
Calculated MW:15kDa
Observed MW:14kDa
Synonyms:SNRNP25, C16orf33
Background:Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 25K protein that is a component of the U12-type spliceosome.
UniProt Protein Function:SNRNP25: Component of the U11/U12 snRNPs that are part of the U12- type spliceosome.
UniProt Protein Details:

Protein type:RNA splicing; RNA processing

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; intercellular bridge; cytoplasm; nucleus; U12-dependent spliceosome

Biological Process: RNA splicing; mRNA processing

NCBI Summary:Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 25K protein that is a component of the U12-type spliceosome. [provided by RefSeq, Apr 2010]
UniProt Code:Q9BV90
NCBI GenInfo Identifier:12655062
NCBI Gene ID:79622
NCBI Accession:BC001381
UniProt Secondary Accession:Q9BV90,Q1W6H3, Q6IEF8, Q9H5W4,
UniProt Related Accession:Q9BV90
Molecular Weight:15,270 Da
NCBI Full Name:Homo sapiens small nuclear ribonucleoprotein 25kDa (U11/U12), mRNA
NCBI Synonym Full Names:small nuclear ribonucleoprotein U11/U12 subunit 25
NCBI Official Symbol:SNRNP25  
NCBI Official Synonym Symbols:C16orf33  
NCBI Protein Information:U11/U12 small nuclear ribonucleoprotein 25 kDa protein
UniProt Protein Name:U11/U12 small nuclear ribonucleoprotein 25 kDa protein
UniProt Synonym Protein Names:Minus-99 protein
Protein Family:U11/U12 small nuclear ribonucleoprotein 25 kDa protein
UniProt Gene Name:SNRNP25  
UniProt Entry Name:SNR25_HUMAN
Anti-SNRNP25 Antibody (CAB15511)Western blot analysis of extracts of various cell lines, using SNRNP25 antibody (CAB15511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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