Anti-SMYD2 Antibody (CAB6474)

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SKU:
CAB6474
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-SMYD2 Antibody
Antibody SKU:CAB6474
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse
Positive Samples:HeLa, 293T, Mouse heart, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRAE GLGG LERF CSPG KGRG LRAL QPFQ VGDL LFSC PAYA YVLT VNER GNHC EYCF TRKE GLSK CGRC KQAF YCNV ECQK EDWP MHKL ECSP MVVF GENW NPSE TVRL TARI LAKQ KIHP ERTP SEKL LAVK EFES HLDK LDNE KKDL IQSD IAAL HHFY SKHL GFPD NDSL VVLF AQVN CNGF TIED EELS HLGS AIFP DVAL MNHS CCPN VIVT YKGT LAEV RAVQ EIKP GEEV FTSY IDLL YPTE DRND RLRD SYFF TCEC QECT TK
Gene ID:56950
Uniprot:Q9NRG4
Cellular Location:Cytoplasm, Nucleus, cytosol
Calculated MW:30kDa/49kDa
Observed MW:50kDa
Synonyms:SMYD2, HSKM-B, KMT3C, ZMYND14
Background:
UniProt Protein Function:SMYD2: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1. Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1. Expression is repressed by CEBPA.
UniProt Protein Details:

Protein type:Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function:protein binding; p53 binding; metal ion binding; protein-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; histone H3-K36 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of DNA damage response, signal transduction by p53 class mediator

NCBI Summary:SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]
UniProt Code:Q9NRG4
NCBI GenInfo Identifier:90185234
NCBI Gene ID:56950
NCBI Accession:Q9NRG4.2
UniProt Related Accession:Q9NRG4
Molecular Weight:30kDa; 49kDa
NCBI Full Name:N-lysine methyltransferase SMYD2
NCBI Synonym Full Names:SET and MYND domain containing 2
NCBI Official Symbol:SMYD2  
NCBI Official Synonym Symbols:KMT3C; HSKM-B; ZMYND14  
NCBI Protein Information:N-lysine methyltransferase SMYD2
UniProt Protein Name:N-lysine methyltransferase SMYD2
UniProt Synonym Protein Names:HSKM-B; Histone methyltransferase SMYD2 (EC:2.1.1.43); Lysine N-methyltransferase 3C; SET and MYND domain-containing protein 2
Protein Family:N-lysine methyltransferase
UniProt Gene Name:SMYD2  
UniProt Entry Name:SMYD2_HUMAN
Anti-SMYD2 Antibody (CAB6474)Western blot analysis of extracts of various cell lines, using SMYD2 antibody (CAB6474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-SMYD2 Antibody (CAB6474)Immunofluorescence analysis of U2OS cells using SMYD2 antibody (CAB6474). Blue: DAPI for nuclear staining.

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