Anti-SMPD4 Antibody (CAB15473)
- SKU:
- CAB15473
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SMPD4 Antibody |
| Antibody SKU: | CAB15473 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, |
| Positive Samples: | A-549 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ITQA KHTA KSIS DQCA ESPA GHSF LSWL GFSS MDTN GSYT ANDL DEMG QDSV RKTD EYLE KALE YLRQ IFRL SEAQ LRQF TLAL GTTQ DENG KKQL PDCI VGED GLIL TPLG RYQI INGL RRFE IEYQ GDPE LQPI RSYE IASL VRTL FRLS SAIN HRFA GQMA ALCS RDDF LGSF CRYH LTEP GLAS RHLL SPVG RRQV A |
| Gene ID: | 55627 |
| Uniprot: | Q9NXE4 |
| Cellular Location: | Endoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass membrane protein |
| Calculated MW: | 44kDa, 56-69kDa, 83-98kDa |
| Observed MW: | 100kDa |
| Synonyms: | SMPD4, NET13, NSMASE-3, NSMASE3 |
| Background: | The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. |
| UniProt Protein Function: | SMPD4: Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid Metabolism - sphingolipid; Endoplasmic reticulum; EC 3.1.4.12; Membrane protein, integral; Phosphodiesterase Chromosomal Location of Human Ortholog: 2q21.1 Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; trans-Golgi network Molecular Function:sphingomyelin phosphodiesterase activity; metal ion binding; sphingomyelin phosphodiesterase D activity Biological Process: sphingolipid metabolic process; glycerophospholipid catabolic process; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process |
| NCBI Summary: | Sphingomyelinases (EC 3.1.4.12), such as SMPD4, catalyze the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide (Krut et al., 2006 [PubMed 16517606]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9NXE4 |
| NCBI GenInfo Identifier: | 102467484 |
| NCBI Gene ID: | 55627 |
| NCBI Accession: | NP_060221.2 |
| UniProt Secondary Accession: | Q9NXE4,Q6FI76, Q6P1P7, Q6ZT43, Q9H0M2, B4DM23, B4DQ31 B4DRB8, B4DWK7, B4E0L6, E7ESA2, E9PCE6, |
| UniProt Related Accession: | Q9NXE4 |
| Molecular Weight: | 60,173 Da |
| NCBI Full Name: | sphingomyelin phosphodiesterase 4 isoform 1 |
| NCBI Synonym Full Names: | sphingomyelin phosphodiesterase 4 |
| NCBI Official Symbol: | SMPD4 |
| NCBI Official Synonym Symbols: | NET13; NSMASE3; NSMASE-3 |
| NCBI Protein Information: | sphingomyelin phosphodiesterase 4 |
| UniProt Protein Name: | Sphingomyelin phosphodiesterase 4 |
| UniProt Synonym Protein Names: | Neutral sphingomyelinase 3; nSMase-3; nSMase3; Neutral sphingomyelinase III |
| Protein Family: | Sphingomyelin phosphodiesterase |
| UniProt Gene Name: | SMPD4 |
| UniProt Entry Name: | NSMA3_HUMAN |
 | Western blot analysis of extracts of A-549 cells, using SMPD4 antibody (CAB15473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Immunofluorescence analysis of L929 cells using SMPD4 Rabbit pAb (CAB15473) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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