Anti-Smad6 Antibody (CAB14749)

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SKU:
CAB14749
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-Smad6 Antibody
Antibody SKU:CAB14749
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 257-496 of human Smad6 (NP_005576.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, 22Rv1, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 257-496 of human Smad6 (NP_005576.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PTVC CNPY HFSR LCGP ESPP PPYS RLSP RDEY KPLD LSDS TLSY TETE ATNS LITA PGEF SDAS MSPD ATKP SHWC SVAY WEHR TRVG RLYA VYDQ AVSI FYDL PQGS GFCL GQLN LEQR SESV RRTR SKIG FGIL LSKE PDGV WAYN RGEH PIFV NSPT LDAP GGRA LVVR KVPP GYSI KVFD FERS GLQH APEP DAAD GPYD PNSV RISF AKGW GPCY SRQF ITSC PCWL EILL NNPR
Gene ID:4091
Uniprot:O43541
Cellular Location:Nucleus
Calculated MW:26kDa/35kDa/53kDa
Observed MW:53kDa
Synonyms:SMAD6, AOVD2, HsT17432, MADH6, MADH7
Background:The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.
UniProt Protein Function:SMAD6: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. Interacts with NEDD4L. Interacts with WWP1. Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX. Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. Belongs to the dwarfin/SMAD family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: transcription factor complex; protein complex; cytoplasm; cytosol; nucleus

Molecular Function:identical protein binding; protein binding; ubiquitin protein ligase binding; metal ion binding; chromatin binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; transcription factor activity

Biological Process: BMP signaling pathway; fat cell differentiation; negative regulation of cell proliferation; zygotic determination of dorsal/ventral axis; transcription, DNA-dependent; ureteric bud development; response to estrogen stimulus; transforming growth factor beta receptor signaling pathway; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; cell-substrate adhesion; negative regulation of apoptosis; negative regulation of BMP signaling pathway

Disease: Aortic Valve Disease 2

NCBI Summary:The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]
UniProt Code:O43541
NCBI GenInfo Identifier:115502451
NCBI Gene ID:4091
NCBI Accession:O43541.2
UniProt Related Accession:O43541
Molecular Weight:
NCBI Full Name:Mothers against decapentaplegic homolog 6
NCBI Synonym Full Names:SMAD family member 6
NCBI Official Symbol:SMAD6  
NCBI Official Synonym Symbols:AOVD2; MADH6; MADH7; HsT17432  
NCBI Protein Information:mothers against decapentaplegic homolog 6
UniProt Protein Name:Mothers against decapentaplegic homolog 6
UniProt Synonym Protein Names:SMAD family member 6; SMAD 6; Smad6; hSMAD6
Protein Family:Mothers against decapentaplegic
UniProt Gene Name:SMAD6  
UniProt Entry Name:SMAD6_HUMAN
Anti-Smad6 Antibody (CAB14749)Western blot analysis of extracts of various cell lines, using Smad6 antibody (CAB14749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-Smad6 Antibody (CAB14749)Immunohistochemistry of paraffin-embedded rat brain using Smad6 antibody (CAB14749) at dilution of 1:100 (40x lens).
Anti-Smad6 Antibody (CAB14749)Immunofluorescence analysis of NIH-3T3 cells using Smad6 antibody (CAB14749) at dilution of 1:100. Blue: DAPI for nuclear staining.

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