Anti-SLC12A5 Antibody (CAB15486)
- SKU:
- CAB15486
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-SLC12A5 Antibody |
| Antibody SKU: | CAB15486 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 945-1025 of human SLC12A5 (NP_001128243.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:200 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 945-1025 of human SLC12A5 (NP_001128243.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TKNE RERE IQSI TDES RGSI RRKN PANT RLRL NVPE ETAG DSEE KPEE EVQL IHDQ SAPS CPSS SPSP GEEP EGEG ETDP E |
| Gene ID: | 57468 |
| Uniprot: | Q9H2X9 |
| Cellular Location: | Membrane, Multi-pass membrane protein |
| Calculated MW: | 123kDa/126kDa |
| Observed MW: | 110-260kDa |
| Synonyms: | SLC12A5, EIEE34, EIG14, KCC2, hKCC2 |
| Background: | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | KCC2: Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1. Brain specific. Detected in neuronal cells. Inhibited by WNK3. Belongs to the SLC12A transporter family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transporter, SLC family; Membrane protein, multi-pass; Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: cell soma; integral to plasma membrane; integral to membrane; plasma membrane; dendritic shaft Molecular Function:protein kinase binding; potassium:chloride symporter activity Biological Process: response to drug; synaptic transmission; transport; cellular ion homeostasis; multicellular organism growth; ion transport; learning; transmembrane transport; potassium ion transport; thermosensory behavior |
| NCBI Summary: | K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Sep 2008] |
| UniProt Code: | Q9H2X9 |
| NCBI GenInfo Identifier: | 161784306 |
| NCBI Gene ID: | 57468 |
| NCBI Accession: | Q9H2X9.3 |
| UniProt Related Accession: | Q9H2X9 |
| Molecular Weight: | |
| NCBI Full Name: | Solute carrier family 12 member 5 |
| NCBI Synonym Full Names: | solute carrier family 12 member 5 |
| NCBI Official Symbol: | SLC12A5 |
| NCBI Official Synonym Symbols: | KCC2; EIG14; hKCC2; EIEE34 |
| NCBI Protein Information: | solute carrier family 12 member 5 |
| UniProt Protein Name: | Solute carrier family 12 member 5 |
| UniProt Synonym Protein Names: | Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter |
| Protein Family: | Solute carrier family |
| UniProt Gene Name: | SLC12A5 |
| UniProt Entry Name: | S12A5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SLC12A5 antibody (CAB15486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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