Anti-SLC12A2 Antibody (CAB11675)
- SKU:
- CAB11675
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| Antibody Name: | Anti-SLC12A2 Antibody |
| Antibody SKU: | CAB11675 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human SLC12A2 (NP_001037.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse pancreas, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human SLC12A2 (NP_001037.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GEAS GESE PAKG SEEA KGRF RVNF VDPA ASSS AEDS LSDA AGVG VDGP NVSF QNGG DTVL SEGS SLHS GGGG GSGH HQHY YYDT HTNT YYLR TFGH NTMD AVPR IDHY RHTA AQLG EKLL RPSL AELH DELE KEPF EDGF ANGE ESTP TRDA VVTY TAES K |
| Gene ID: | 6558 |
| Uniprot: | P55011 |
| Cellular Location: | Membrane, Multi-pass membrane protein |
| Calculated MW: | 129kDa/131kDa |
| Observed MW: | 170kDa |
| Synonyms: | SLC12A2, BSC, BSC2, NKCC1, PPP1R141 |
| Background: | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. |
| UniProt Protein Function: | NKCC1: a multi-pass membrane cation transporter protein. Aids transcellular movement of chloride across both secretory and absorptive epithelia. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Expressed in many tissues. Bumetanide is a potent NKCC1 antagonist. Two alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter Chromosomal Location of Human Ortholog: 5q23.3 Cellular Component: membrane; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle Molecular Function:protein binding; ammonium transmembrane transporter activity; sodium:potassium:chloride symporter activity Biological Process: hyperosmotic response; transport; multicellular organism growth; transepithelial chloride transport; sodium ion transport; ion transport; detection of mechanical stimulus involved in sensory perception of sound; positive regulation of cell volume; transmembrane transport; gamma-aminobutyric acid signaling pathway; potassium ion transport; ammonium transport |
| NCBI Summary: | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| UniProt Code: | P55011 |
| NCBI GenInfo Identifier: | 1709292 |
| NCBI Gene ID: | 6558 |
| NCBI Accession: | P55011.1 |
| UniProt Secondary Accession: | P55011,P55012, |
| UniProt Related Accession: | P55011 |
| Molecular Weight: | 1212 |
| NCBI Full Name: | Solute carrier family 12 member 2 |
| NCBI Synonym Full Names: | solute carrier family 12 (sodium/potassium/chloride transporter), member 2 |
| NCBI Official Symbol: | SLC12A2 |
| NCBI Official Synonym Symbols: | BSC; BSC2; NKCC1; PPP1R141 |
| NCBI Protein Information: | solute carrier family 12 member 2; basolateral Na-K-Cl symporter; protein phosphatase 1, regulatory subunit 141; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; solute carrier family 12 (sodium/potassium/chloride transporters), member 2 |
| UniProt Protein Name: | Solute carrier family 12 member 2 |
| UniProt Synonym Protein Names: | Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1 |
| UniProt Gene Name: | SLC12A2 |
| UniProt Entry Name: | S12A2_HUMAN |
 | Western blot analysis of extracts of mouse pancreas, using SLC12A2 antibody (CAB11675) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Western blot analysis of extracts of rat brain, using SLC12A2 antibody (CAB11675) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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