Anti-SIRT5 Antibody (CAB5784)[KO Validated]

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SKU:
CAB5784
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-SIRT5 Antibody [KO Validated]
Antibody SKU:CAB5784
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human SIRT5 (NP_036373.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human SIRT5 (NP_036373.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRPL QIVP SRLI SQLY CGLK PPAS TRNQ ICLK MARP SSSM ADFR KFFA KAKH IVII SGAG VSAE SGVP TFRG AGGY WRKW QAQD LATP LAFA HNPS RVWE FYHY RREV MGSK EPNA GHRA IAEC ETRL GKQG RRVV VITQ NIDE LHRK AGTK NLLE IHGS LFKT RCTS CGVV AENY KSPI CPAL SGKG APEP GTQD ASIP VEKL PRCE EAGC GGLL RPHV VWFG ENLD PAIL EEVD RELA HCDL CLVV GTSS VVYP AAMF APQV AARG VPVA EFNT ETTP ATNR FRFH FQGP CGTT LPEA LACH ENET VS
Gene ID:23408
Uniprot:Q9NXA8
Cellular Location:Cytoplasm, Mitochondrion, Mitochondrion intermembrane space, Mitochondrion matrix, Nucleus, cytosol
Calculated MW:21kDa/31kDa/32kDa/33kDa
Observed MW:30KDa
Synonyms:SIRT5, SIR2L5, sirtuin 5
Background:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants.
UniProt Protein Function:SIRT5: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD- dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. Belongs to the sirtuin family. Class III subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; EC 3.5.1.-; Deacetylase

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial intermembrane space; cytosol; nucleus

Molecular Function:zinc ion binding; NAD+ ADP-ribosyltransferase activity

Biological Process: protein amino acid ADP-ribosylation; protein amino acid deacetylation; chromatin silencing

NCBI Summary:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010]
UniProt Code:Q9NXA8
NCBI GenInfo Identifier:38258652
NCBI Gene ID:23408
NCBI Accession:Q9NXA8.2
UniProt Secondary Accession:Q9NXA8,Q5T294, Q5T295, Q9Y6E6, B4DFM4, B4DYJ5, F5H5Z9
UniProt Related Accession:Q9NXA8
Molecular Weight:310
NCBI Full Name:NAD-dependent protein deacylase sirtuin-5, mitochondrial
NCBI Synonym Full Names:sirtuin 5
NCBI Official Symbol:SIRT5  
NCBI Official Synonym Symbols:SIR2L5  
NCBI Protein Information:NAD-dependent protein deacylase sirtuin-5, mitochondrial; SIRT5; sir2-like 5; sirtuin type 5; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial
UniProt Protein Name:NAD-dependent protein deacylase sirtuin-5, mitochondrial
UniProt Synonym Protein Names:Regulatory protein SIR2 homolog 5; SIR2-like protein 5
Protein Family:NAD-dependent protein deacylase sirtuin
UniProt Gene Name:SIRT5  
UniProt Entry Name:SIR5_HUMAN
Anti-SIRT5 Antibody [KO Validated] (CAB5784)Western blot analysis of extracts of 293T cells, using SIRT5 antibody (CAB5784) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

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