Anti-SHMT1 Antibody (CAB12489)
- SKU:
- CAB12489
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-SHMT1 Antibody |
| Antibody SKU: | CAB12489 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, HT-29, K-562, A375, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FKVY QHQV VANC RALS EALT ELGY KIVT GGSD NHLI LVDL RSKG TDGG RAEK VLEA CSIA CNKN TCPG DRSA LRPS GLRL GTPA LTSR GLLE KDFQ KVAH FIHR GIEL TLQI QSDT GVRA TLKE FKER LAGD KYQA AVQA LREE VESF ASLF PLPG LPDF |
| Gene ID: | 6470 |
| Uniprot: | P34896 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 37kDa/44kDa/49kDa/53kDa |
| Observed MW: | 53kDa |
| Synonyms: | SHMT1, CSHMT, SHMT |
| Background: | This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | SHMT1: Interconversion of serine and glycine. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.1; Amino Acid Metabolism - glycine, serine and threonine; Energy Metabolism - methane; Other Amino Acids Metabolism - cyanoamino acid; Lyase; Methyltransferase; Mitochondrial Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus Molecular Function:L-allo-threonine aldolase activity; amino acid binding; protein homodimerization activity; glycine hydroxymethyltransferase activity; pyridoxal phosphate binding Biological Process: purine base biosynthetic process; vitamin metabolic process; folic acid metabolic process; L-serine catabolic process; glycine biosynthetic process from serine; water-soluble vitamin metabolic process; carnitine biosynthetic process; protein tetramerization; protein homotetramerization |
| NCBI Summary: | This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| UniProt Code: | P34896 |
| NCBI GenInfo Identifier: | 462184 |
| NCBI Gene ID: | 6470 |
| NCBI Accession: | P34896.1 |
| UniProt Related Accession: | P34896 |
| Molecular Weight: | |
| NCBI Full Name: | Serine hydroxymethyltransferase, cytosolic |
| NCBI Synonym Full Names: | serine hydroxymethyltransferase 1 |
| NCBI Official Symbol: | SHMT1 |
| NCBI Official Synonym Symbols: | SHMT; CSHMT |
| NCBI Protein Information: | serine hydroxymethyltransferase, cytosolic |
| UniProt Protein Name: | Serine hydroxymethyltransferase, cytosolic |
| UniProt Synonym Protein Names: | Glycine hydroxymethyltransferase; Serine methylase |
| Protein Family: | Serine hydroxymethyltransferase |
| UniProt Gene Name: | SHMT1 |
| UniProt Entry Name: | GLYC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SHMT1 antibody (CAB12489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using SHMT1 antibody (CAB12489) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using SHMT1 antibody (CAB12489) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U-2 OS cells using SHMT1 antibody (CAB12489) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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