Anti-SGMS1 Antibody (CAB15008)

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SKU:
CAB15008
Product type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
Frequently bought together:

Description

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Antibody Name:Anti-SGMS1 Antibody
Antibody SKU:CAB15008
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human SGMS1 (NP_671512.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:A-549
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human SGMS1 (NP_671512.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MKEV VYWS PKKV ADWL LENA MPEY CEPL EHFT GQDL INLT QEDF KKPP LCRV SSDN GQRL LDMI ETLK MEHH LEAH KNGH ANGH LNIG VDIP TPDG SFSI KIKP NGMP NGYR KEMI KIPM PELE RSQY PMEW GK
Gene ID:259230
Uniprot:Q86VZ5
Cellular Location:Golgi apparatus membrane, Multi-pass membrane protein
Calculated MW:25kDa/49kDa
Observed MW:49kDa
Synonyms:SGMS1, MOB, MOB1, SMS1, TMEM23, hmob33
Background:The protein encoded by this gene is predicted to be a five-pass transmembrane protein. This gene may be predominately expressed in brain.
UniProt Protein Function:TMEM23: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide. Belongs to the sphingomyelin synthase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; EC 2.7.8.27; Membrane protein, integral; Lipid Metabolism - sphingolipid; Transferase

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: Golgi membrane; membrane; endoplasmic reticulum; Golgi trans cisterna; plasma membrane; integral to Golgi membrane; nucleus

Molecular Function:sphingomyelin synthase activity; kinase activity; ceramide cholinephosphotransferase activity

Biological Process: sphingolipid metabolic process; sphingolipid biosynthetic process; apoptosis; cell growth; sphingomyelin biosynthetic process; inflammatory response; phosphorylation

NCBI Summary:The protein encoded by this gene is predicted to be a five-pass transmembrane protein. This gene may be predominately expressed in brain. [provided by RefSeq, Jul 2008]
UniProt Code:Q86VZ5
NCBI GenInfo Identifier:44888473
NCBI Gene ID:259230
NCBI Accession:Q86VZ5.2
UniProt Secondary Accession:Q86VZ5,Q68U43, Q6EKK0, Q75SP1,
UniProt Related Accession:Q86VZ5
Molecular Weight:419
NCBI Full Name:Phosphatidylcholine:ceramide cholinephosphotransferase 1
NCBI Synonym Full Names:sphingomyelin synthase 1
NCBI Official Symbol:SGMS1  
NCBI Official Synonym Symbols:MOB; MOB1; SMS1; TMEM23; hmob33  
NCBI Protein Information:phosphatidylcholine:ceramide cholinephosphotransferase 1; protein Mob; transmembrane protein 23; medulla oblongata-derived protein
UniProt Protein Name:Phosphatidylcholine:ceramide cholinephosphotransferase 1
UniProt Synonym Protein Names:Medulla oblongata-derived protein; Protein Mob; Sphingomyelin synthase 1; Transmembrane protein 23
UniProt Gene Name:SGMS1  
UniProt Entry Name:SMS1_HUMAN
Anti-SGMS1 Antibody (CAB15008)Western blot analysis of extracts of A-549 cells, using SGMS1 antibody (CAB15008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 930s.
Anti-SGMS1 Antibody (CAB15008)Immunofluorescence analysis of HeLa cells using SGMS1 Rabbit pAb (CAB15008) at dilution of 1:100. Blue: DAPI for nuclear staining.

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