Anti-SFTPC Antibody (CAB1835)
- SKU:
- CAB1835
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SFTPC Antibody |
| Antibody SKU: | CAB1835 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human SFTPC (NP_001165881.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-197 of human SFTPC (NP_001165881.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDVG SKEV LMES PPDY SAAP RGRF GIPC CPVH LKRL LIVV VVVV LIVV VIVG ALLM GLHM SQKH TEMV LEMS IGAP EAQQ RLAL SEHL VTTA TFSI GSTG LVVY DYQQ LLIA YKPA PGTC CYIM KIAP ESIP SLEA LTRK VHNF QMEC SLQA KPAV PTSK LGQA EGRD AGSA PSGG DPAF LGMA VSTL CGEV PLYY I |
| Gene ID: | 6440 |
| Uniprot: | P11686 |
| Cellular Location: | Secreted, extracellular space, surface film |
| Calculated MW: | 20kDa/21kDa |
| Observed MW: | 21kDa |
| Synonyms: | SFTPC, BRICD6, PSP-C, SFTP2, SMDP2, SP-C |
| Background: | This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified. |
| UniProt Protein Function: | SFTPC: Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2); also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS); also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 8p21 Cellular Component: multivesicular body; extracellular space Molecular Function:protein binding; protein homodimerization activity Biological Process: circadian rhythm; response to cAMP; response to hyperoxia; response to retinoic acid; response to glucocorticoid stimulus; response to glucose stimulus; response to lipopolysaccharide; respiratory gaseous exchange; protein homooligomerization; response to vitamin A Disease: Pulmonary Fibrosis, Idiopathic; Surfactant Metabolism Dysfunction, Pulmonary, 2 |
| NCBI Summary: | This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.[provided by RefSeq, Feb 2010] |
| UniProt Code: | P11686 |
| NCBI GenInfo Identifier: | 131425 |
| NCBI Gene ID: | 6440 |
| NCBI Accession: | P11686.2 |
| UniProt Related Accession: | P11686 |
| Molecular Weight: | |
| NCBI Full Name: | Pulmonary surfactant-associated protein C |
| NCBI Synonym Full Names: | surfactant protein C |
| NCBI Official Symbol: | SFTPC |
| NCBI Official Synonym Symbols: | SP-C; PSP-C; SFTP2; SMDP2; BRICD6 |
| NCBI Protein Information: | pulmonary surfactant-associated protein C |
| UniProt Protein Name: | Pulmonary surfactant-associated protein C |
| UniProt Synonym Protein Names: | Pulmonary surfactant-associated proteolipid SPL(Val); SP5 |
| UniProt Gene Name: | SFTPC |
| UniProt Entry Name: | PSPC_HUMAN |
 | Western blot analysis of extracts of Mouse lung, using SFTPC antibody (CAB1835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human lung using SFTPC antibody (CAB1835) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat lung using SFTPC antibody (CAB1835) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using SFTPC antibody (CAB1835) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of rat lung using SFTPC Rabbit pAb (CAB1835) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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