Anti-SFRP5 Antibody (CAB16734)
- SKU:
- CAB16734
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-SFRP5 Antibody |
| Antibody SKU: | CAB16734 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-317 of human SFRP5 (NP_003006.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-317 of human SFRP5 (NP_003006.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EEYD YYGW QAEP LHGR SYSK PPQC LDIP ADLP LCHT VGYK RMRL PNLL EHES LAEV KQQA SSWL PLLA KRCH SDTQ VFLC SLFA PVCL DRPI YPCR SLCE AVRA GCAP LMEA YGFP WPEM LHCH KFPL DNDL CIAV QFGH LPAT APPV TKIC AQCE MEHS ADGL MEQM CSSD FVVK MRIK EIKI ENGD RKLI GAQK KKKL LKPG PLKR KDTK RLVL HMKN GAGC PCPQ LDSL AGSF LVMG RKVD GQLL LMAV YRWD KKNK EMKF AVKF MFSY PCSL YYPF FYGA AEPH |
| Gene ID: | 6425 |
| Uniprot: | Q5T4F7 |
| Cellular Location: | |
| Calculated MW: | 35kDa |
| Observed MW: | 32kDa |
| Synonyms: | SFRP5, SARP3 |
| Background: | Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. |
| UniProt Code: | Q5T4F7 |
| NCBI GenInfo Identifier: | 188528609 |
| NCBI Gene ID: | 6425 |
| NCBI Accession: | NP_003006.2 |
| UniProt Secondary Accession: | Q5T4F7,O14780, Q86TH7, |
| UniProt Related Accession: | Q5T4F7 |
| Molecular Weight: | 35,563 Da |
| NCBI Full Name: | secreted frizzled-related protein 5 |
| NCBI Synonym Full Names: | secreted frizzled-related protein 5 |
| NCBI Official Symbol: | SFRP5 |
| NCBI Official Synonym Symbols: | SARP3 |
| NCBI Protein Information: | secreted frizzled-related protein 5; FRP-1b; SARP-3; sFRP-5; frizzled-related protein 1b; secreted apoptosis related protein 3; secreted apoptosis-related protein 3 |
| UniProt Protein Name: | Secreted frizzled-related protein 5 |
| UniProt Synonym Protein Names: | Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3 |
| Protein Family: | Secreted frizzled-related protein |
| UniProt Gene Name: | SFRP5 |
| UniProt Entry Name: | SFRP5_HUMAN |
 | Western blot analysis of extracts of Mouse pancreas, using SFRP5 antibody (CAB16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of mouse pancreas using SFRP5 Polyclonal Antibody (CAB16734) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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