Anti-SF3B1 항체 (CAB15801)

Antibody Name:	Anti-SF3B1 Antibody
Antibody SKU:	CAB15801
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Jurkat, HeLa, NIH/3T3, Mouse brain, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAKI AKTH EDIE AQIR EIQG KKAA LDEA QGVG LDST GYYD QEIY GGSD SRFA GYVT SIAA TELE DDDD DYSS STSL LGQK KPGY HAPV ALLN DIPQ STEQ YDPF AEHR PPKI ADRE DEYK KHRR TMII SPER LDPF ADGF YSAA
Gene ID:	23451
Uniprot:	O75533
Cellular Location:	Nucleus speckle
Calculated MW:	16kDa/145kDa
Observed MW:	160kDa

Synonyms:

SF3B1, Hsh155, MDS, PRP10, PRPF10, SAP155, SF3b155

Background:

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	SF3B1: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (bps) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Phosphorylated during the splicing catalytic steps.
UniProt Protein Details:	Protein type:RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 2q33.1 Cellular Component: nucleoplasm; nucleus; snRNP U2; U12-dependent spliceosome Molecular Function:mRNA binding; protein binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of gene expression, epigenetic; spliceosome assembly Disease: Myelodysplastic Syndrome
NCBI Summary:	This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O75533
NCBI GenInfo Identifier:	269849656
NCBI Gene ID:	23451
NCBI Accession:	O75533.3
UniProt Secondary Accession:	O75533,E9PCH3,
UniProt Related Accession:	O75533
Molecular Weight:	16,020 Da
NCBI Full Name:	Splicing factor 3B subunit 1
NCBI Synonym Full Names:	splicing factor 3b subunit 1
NCBI Official Symbol:	SF3B1
NCBI Official Synonym Symbols:	MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155
NCBI Protein Information:	splicing factor 3B subunit 1
UniProt Protein Name:	Splicing factor 3B subunit 1
UniProt Synonym Protein Names:	Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155
Protein Family:	Splicing factor
UniProt Gene Name:	SF3B1
UniProt Entry Name:	SF3B1_HUMAN

	Western blot analysis of extracts of various cell lines, using SF3B1 antibody (CAB15801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U-2 OS cells using SF3B1 antibody (CAB15801) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-Phospho-SF3B1-T313 pAb Antibody (CABP0844)

SF3B1 Antibody (PACO01757)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)