Anti-SETD8 Antibody (CAB7305)

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SKU:
CAB7305
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-SETD8 Antibody
Antibody SKU:CAB7305
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 73-352 of human SETD8 (NP_065115.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, 293T, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 73-352 of human SETD8 (NP_065115.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ENSV THHE VKCQ GKPL AGIY RKRE EKRN AGNA VRSA MKSE EQKI KDAR KGPL VPFP NQKS EAAE PPKT PPSS CDST NAAI AKQA LKKP IKGK QAPR KKAQ GKTQ QNRK LTDF YPVR RSSR KSKA ELQS EERK RIDE LIES GKEE GMKI DLID GKGR GVIA TKQF SRGD FVVE YHGD LIEI TDAK KREA LYAQ DPST GCYM YYFQ YLSK TYCV DATR ETNR LGRL INHS KCGN CQTK LHDI DGVP HLIL IASR DIAA GEEL LYDY GDRS KASI EAHP WLKH
Gene ID:387893
Uniprot:Q9NQR1
Cellular Location:Chromosome, Nucleus
Calculated MW:39kDa/42kDa
Observed MW:43kDa
Synonyms:KMT5A, PR-Set7, SET07, SET8, SETD8
Background:
UniProt Protein Function:SETD8: a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Methylates K20 of histone H4 (H4K20me1), a specific tag for epigenetic transcriptional repression. SETD8 protein and H4K20me1 levels are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. Interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Nucleosomes are preferred as substrate compared to free histones. Methylates p53K382, leading to repression of the pro-apoptotic and checkpoint activation functions of p53. SET8 expression levels decrease in response to DNA damage, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4K20 and p53K382 appear to be important for the functions of SET8 in S phase. Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; nucleolus; chromosome; nucleus

Molecular Function:protein binding; p53 binding; protein-lysine N-methyltransferase activity; transcription corepressor activity; histone-lysine N-methyltransferase activity

Biological Process: mitosis; transcription, DNA-dependent; cell division; peptidyl-lysine mono-methylation; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; regulation of DNA damage response, signal transduction by p53 class mediator

UniProt Code:Q9NQR1
NCBI GenInfo Identifier:25091219
NCBI Gene ID:387893
NCBI Accession:Q9NQR1.3
UniProt Secondary Accession:Q9NQR1,Q86W83, Q8TD09, A8K9D0,
UniProt Related Accession:Q9NQR1
Molecular Weight:393
NCBI Full Name:N-lysine methyltransferase SETD8
NCBI Synonym Full Names:SET domain containing (lysine methyltransferase) 8
NCBI Official Symbol:SETD8  
NCBI Official Synonym Symbols:SET8; KMT5A; SET07; PR-Set7  
NCBI Protein Information:N-lysine methyltransferase SETD8; PR/SET07; H4-K20-HMTase SETD8; lysine N-methyltransferase 5A; SET domain-containing protein 8; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; histone-lysine N-methyltransferase SETD8; H4-K20-spec
UniProt Protein Name:N-lysine methyltransferase SETD8
UniProt Synonym Protein Names:H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8 (EC:2.1.1.43); Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; PR-Set7; PR/SET07; SET domain-containing protein 8
Protein Family:N-lysine methyltransferase
UniProt Gene Name:SETD8  
UniProt Entry Name:SETD8_HUMAN
Anti-SETD8 Antibody (CAB7305)Western blot analysis of extracts of various cell lines, using SETD8 antibody (CAB7305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Anti-SETD8 Antibody (CAB7305)Immunofluorescence analysis of MCF-7 cells using SETD8 antibody (CAB7305).

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