Anti-SERPINC1 항체 (CAB1574)

Antibody Name:	Anti-SERPINC1 Antibody
Antibody SKU:	CAB1574
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-185 of human SERPINC1 (NP_000479.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	U-251MG, HepG2, PC-3, HO-8910, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 33-185 of human SERPINC1 (NP_000479.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HGSP VDIC TAKP RDIP MNPM CIYR SPEK KATE DEGS EQKI PEAT NRRV WELS KANS RFAT TFYQ HLAD SKND NDNI FLSP LSIS TAFA MTKL GACN DTLQ QLME VFKF DTIS EKTS DQIH FFFA KLNC RLYR KANK SSKL VSAN RLFG DKSL T
Gene ID:	462
Uniprot:	P01008
Cellular Location:	Secreted, extracellular space
Calculated MW:	52kDa
Observed MW:	60kDa

Synonyms:

SERPINC1, AT3, AT3D, ATIII, THPH7

Background:

The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

UniProt Protein Function:	SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Inhibitor Chromosomal Location of Human Ortholog: 1q25.1 Cellular Component: extracellular space; plasma membrane; extracellular region Molecular Function:heparin binding; serine-type endopeptidase inhibitor activity; protein binding; protease binding Biological Process: negative regulation of inflammatory response; blood coagulation; response to nutrient Disease: Antithrombin Iii Deficiency
NCBI Summary:	The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]
UniProt Code:	P01008
NCBI GenInfo Identifier:	113936
NCBI Gene ID:	462
NCBI Accession:	P01008.1
UniProt Secondary Accession:	P01008,P78439, P78447, Q13815, Q5TC78, Q7KZ43, Q7KZ97 Q9UC78, B2R6P0,
UniProt Related Accession:	P01008
Molecular Weight:	464
NCBI Full Name:	Antithrombin-III
NCBI Synonym Full Names:	serpin peptidase inhibitor, clade C (antithrombin), member 1
NCBI Official Symbol:	SERPINC1
NCBI Official Synonym Symbols:	AT3; AT3D; ATIII; THPH7
NCBI Protein Information:	antithrombin-III; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
UniProt Protein Name:	Antithrombin-III
UniProt Synonym Protein Names:	Serpin C1
UniProt Gene Name:	SERPINC1
UniProt Entry Name:	ANT3_HUMAN

	Western blot analysis of extracts of various cell lines, using SERPINC1 antibody (CAB1574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using SERPINC1 antibody (CAB1574). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SERPINC1 Antibody (CAB11249)	Human Antithrombin-III / SERPINC1 ELISA Kit
	Human Antithrombin-III (SERPINC1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)