Anti-Semaphorin-4C Antibody (CAB8225)
- SKU:
- CAB8225
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | SEMA4C Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8225 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 749-833 of human SEMA4C (NP_060259.4). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, BT-474, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 749-833 of human SEMA4C (NP_060259.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HARC QPGG GPPS PPPG IPGQ PLPS PTRL HLGG GRNS NANG YVRL QLGG EDRG GLGH PLPE LADE LRRK LQQR QPLP DSNP EESS V |
| Gene ID: | 54910 |
| Uniprot: | Q9C0C4 |
| Cellular Location: | Cell junction, Cytoplasmic vesicle, Single-pass type I membrane protein, postsynaptic cell membrane, postsynaptic density, secretory vesicle, synapse, synaptic vesicle membrane |
| Calculated MW: | 92kDa |
| Observed MW: | 110kDa |
| Synonyms: | SEMA4C, M-SEMA-F, SEMACL1, SEMAF, SEMAI |
| Background: |
| UniProt Protein Function: | SEMA4C: Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. Belongs to the semaphorin family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 2q11.2 Cellular Component: extracellular space; membrane; postsynaptic density; synaptic vesicle membrane Molecular Function:chemorepellent activity; protein binding; semaphorin receptor binding Biological Process: cell migration in hindbrain; muscle cell differentiation; negative chemotaxis; negative regulation of axon extension involved in axon guidance; neural crest cell migration; neural tube closure; positive regulation of cell migration; positive regulation of stress-activated MAPK cascade |
| UniProt Code: | Q9C0C4 |
| NCBI GenInfo Identifier: | 47606208 |
| NCBI Gene ID: | 54910 |
| NCBI Accession: | Q9C0C4.2 |
| UniProt Secondary Accession: | Q9C0C4,Q32MJ3, Q7Z5X0, |
| UniProt Related Accession: | Q9C0C4 |
| Molecular Weight: | 92,623 Da |
| NCBI Full Name: | Semaphorin-4C |
| NCBI Synonym Full Names: | semaphorin 4C |
| NCBI Official Symbol: | SEMA4C |
| NCBI Official Synonym Symbols: | SEMAF; SEMAI; SEMACL1; M-SEMA-F |
| NCBI Protein Information: | semaphorin-4C |
| UniProt Protein Name: | Semaphorin-4C |
| Protein Family: | Semaphorin |
| UniProt Gene Name: | SEMA4C |
| UniProt Entry Name: | SEM4C_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SEMA4C antibody (CAB8225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using SEMA4C antibody (CAB8225) at dilution of 1:100 (40x lens). |
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