Anti-SCN1B 항체 (CAB10071)

Antibody Name:	Anti-SCN1B Antibody
Antibody SKU:	CAB10071
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human SCN1B (NP_950238.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, A-549, HeLa, MCF7, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human SCN1B (NP_950238.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GGCV EVDS ETEA VYGM TFKI LCIS CKRR SETN AETF TEWT FRQK GTEE FVKI LRYE NEVL QLEE DERF EGRV VWNG SRGT KDLQ DLSI FITN VTYN HSGD YECH VYRL LFFE NYEH NTSV VKKI HIEV VDKG ESGA ACPF TV
Gene ID:	6324
Uniprot:	Q07699
Cellular Location:	Membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	24kDa/30kDa
Observed MW:	30kDa

Synonyms:

SCN1B, ATFB13, BRGDA5, EIEE52, GEFSP1

Background:

Voltage-gated sodium channels are heteromeric proteins that function in the generation and propagation of action potentials in muscle and neuronal cells. They are composed of one alpha and two beta subunits, where the alpha subunit provides channel activity and the beta-1 subunit modulates the kinetics of channel inactivation. This gene encodes a sodium channel beta-1 subunit. Mutations in this gene result in generalized epilepsy with febrile seizures plus, Brugada syndrome 5, and defects in cardiac conduction. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SCN1B: Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. The subunit beta-1 can modulate multiple alpha subunit isoforms from brain, skeletal muscle, and heart. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN1B are the cause of generalized epilepsy with febrile seizures plus type 1 (GEFS+1). Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity. Defects in SCN1B are the cause of Brugada syndrome type 5 (BRGDA5). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN1B (TC 8.A.17) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Channel, sodium Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: voltage-gated sodium channel complex; T-tubule; extracellular region Molecular Function:sodium channel inhibitor activity; sodium channel regulator activity; voltage-gated sodium channel activity Biological Process: synaptic transmission; membrane depolarization; axon guidance; action potential propagation; corticospinal neuron axon guidance; cell adhesion; locomotion; response to pyrethroid; cardiac muscle contraction Disease: Generalized Epilepsy With Febrile Seizures Plus, Type 1; Atrial Fibrillation, Familial, 13; Brugada Syndrome 5
NCBI Summary:	Voltage-gated sodium channels are heteromeric proteins that function in the generation and propagation of action potentials in muscle and neuronal cells. They are composed of one alpha and two beta subunits, where the alpha subunit provides channel activity and the beta-1 subunit modulates the kinetics of channel inactivation. This gene encodes a sodium channel beta-1 subunit. Mutations in this gene result in generalized epilepsy with febrile seizures plus, Brugada syndrome 5, and defects in cardiac conduction. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
UniProt Code:	Q07699
NCBI GenInfo Identifier:	1705868
NCBI Gene ID:	6324
NCBI Accession:	Q07699.1
UniProt Related Accession:	Q07699
Molecular Weight:
NCBI Full Name:	Sodium channel subunit beta-1
NCBI Synonym Full Names:	sodium voltage-gated channel beta subunit 1
NCBI Official Symbol:	SCN1B
NCBI Official Synonym Symbols:	ATFB13; BRGDA5; EIEE52; GEFSP1
NCBI Protein Information:	sodium channel subunit beta-1
UniProt Protein Name:	Sodium channel subunit beta-1
Protein Family:	Sodium channel
UniProt Gene Name:	SCN1B
UniProt Entry Name:	SCN1B_HUMAN

Western blot analysis of extracts of various cell lines, using SCN1B antibody (CAB10071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

SCN1B Antibody (PACO12054)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)