Anti-SCGN Antibody (CAB12897)

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SKU:
CAB12897
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience
Frequently bought together:

Description

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Antibody Name:Anti-SCGN Antibody
Antibody SKU:CAB12897
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-276 of human SCGN (NP_008929.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse eye, Mouse brain, Rat pancreas
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-276 of human SCGN (NP_008929.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDSS REPT LGRL DAAG FWQV WQRF DADE KGYI EEKE LDAF FLHM LMKL GTDD TVMK ANLH KVKQ QFMT TQDA SKDG RIRM KELA GMFL SEDE NFLL LFRR ENPL DSSV EFMQ IWRK YDAD SSGF ISAA ELRN FLRD LFLH HKKA ISEA KLEE YTGT MMKI FDRN KDGR LDLN DLAR ILAL QENF LLQF KMDA CSTE ERKR DFEK IFAY YDVS KTGA LEGP EVDG FVKD MMEL VQPS ISGV DLDK FREI LLRH CDVN KDGK IQKS ELAL CLGL KINP
Gene ID:10590
Uniprot:O76038
Cellular Location:Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Peripheral membrane protein, Secreted, secretory vesicle membrane
Calculated MW:32kDa
Observed MW:27kDa
Synonyms:SCGN, CALBL, DJ501N12.8, SECRET, SEGN, setagin
Background:The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation.
UniProt Protein Function:SCGN:
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 6p22.3-p22.1

Cellular Component: cytosol; neuron projection; nucleus; synapse

Molecular Function:calcium ion binding

Biological Process: cytosolic calcium ion homeostasis

NCBI Summary:The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008]
UniProt Code:O76038
NCBI GenInfo Identifier:21264511
NCBI Gene ID:10590
NCBI Accession:O76038.2
UniProt Secondary Accession:O76038,Q5VV44, Q96QV7, Q9UJF6, A8K0B2,
UniProt Related Accession:O76038
Molecular Weight:32,040 Da
NCBI Full Name:Secretagogin
NCBI Synonym Full Names:secretagogin, EF-hand calcium binding protein
NCBI Official Symbol:SCGN  
NCBI Official Synonym Symbols:SEGN; CALBL; SECRET; setagin; DJ501N12.8  
NCBI Protein Information:secretagogin
UniProt Protein Name:Secretagogin
Protein Family:Secretagogin
UniProt Gene Name:SCGN  
UniProt Entry Name:SEGN_HUMAN
Anti-SCGN Antibody (CAB12897)Western blot analysis of extracts of various cell lines, using SCGN antibody (CAB12897) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SCGN Antibody (CAB12897)Immunohistochemistry of paraffin-embedded Human breast cancer using SCGN Rabbit pAb (CAB12897) at dilution of 1:100 (40x lens).
Anti-SCGN Antibody (CAB12897)Immunohistochemistry of paraffin-embedded Rat pancreas using SCGN Rabbit pAb (CAB12897) at dilution of 1:100 (40x lens).
Anti-SCGN Antibody (CAB12897)Immunofluorescence analysis of L929 cells using SCGN antibody (CAB12897) at dilution of 1:100. Blue: DAPI for nuclear staining.

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