Anti-SARS Antibody (CAB13350)

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SKU:
CAB13350
Product type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
Frequently bought together:

Description

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Antibody Name:Anti-SARS Antibody
Antibody SKU:CAB13350
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SARS (NP_006504.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HepG2, HeLa, U-251MG, 293T, Mouse kidney, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SARS (NP_006504.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVLD LDLF RVDK GGDP ALIR ETQE KRFK DPGL VDQL VKAD SEWR RCRF RADN LNKL KNLC SKTI GEKM KKKE PVGD DESV PENV LSFD DLTA DALA NLKV SQIK KVRL LIDE AILK CDAE RIKL EAER FENL REIG NLLH PSVP ISND EDVD NKVE RIWG DCTV RKKY SHVD LVVM VDGF EGEK GAVV AGSR GYFL KGVL VFLE QALI QYAL RTLG SRGY IPIY TPFF MRKE VMQE VAQL SQFD EELY KVIG KGSE KSDD NSYD EKYL IATS EQPI AALH RDEW LRPE DLPI KYAG LSTC
Gene ID:6301
Uniprot:P49591
Cellular Location:Cytoplasm
Calculated MW:58kDa
Observed MW:59kDa
Synonyms:SARS, SERRS, SERS
Background:This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown.
UniProt Protein Function:SARS: Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
UniProt Protein Details:

Protein type:Translation; EC 6.1.1.11; RNA processing; Ligase

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol

Molecular Function:RNA binding; serine-tRNA ligase activity

Biological Process: selenocysteine metabolic process; seryl-tRNA aminoacylation; translation; tRNA aminoacylation for protein translation; tRNA processing

NCBI Summary:This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown. [provided by RefSeq, Jul 2010]
UniProt Code:P49591
NCBI GenInfo Identifier:19860217
NCBI Gene ID:6301
NCBI Accession:P49591.3
UniProt Secondary Accession:P49591,Q5T5C8, Q9NSE3, B2R6Y9,
UniProt Related Accession:P49591
Molecular Weight:58,777 Da
NCBI Full Name:Serine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:seryl-tRNA synthetase
NCBI Official Symbol:SARS  
NCBI Official Synonym Symbols:SERS; SERRS  
NCBI Protein Information:serine--tRNA ligase, cytoplasmic
UniProt Protein Name:Serine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase
Protein Family:Serine--tRNA ligase
UniProt Gene Name:SARS  
UniProt Entry Name:SYSC_HUMAN
Anti-SARS Antibody (CAB13350)Western blot analysis of extracts of various cell lines, using SARS antibody (CAB13350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SARS Antibody (CAB13350)Immunohistochemistry of paraffin-embedded rat brain using SARS Rabbit pAb (CAB13350) at dilution of 1:100 (40x lens).
Anti-SARS Antibody (CAB13350)Immunohistochemistry of paraffin-embedded human lung cancer using SARS Rabbit pAb (CAB13350) at dilution of 1:100 (40x lens).
Anti-SARS Antibody (CAB13350)Immunohistochemistry of paraffin-embedded mouse spinal cord using SARS Rabbit pAb (CAB13350) at dilution of 1:100 (40x lens).
Anti-SARS Antibody (CAB13350)Immunofluorescence analysis of MCF-7 cells using SARS antibody (CAB13350). Blue: DAPI for nuclear staining.

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