Anti-S100A5 Antibody (CAB14779)
- SKU:
- CAB14779
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-S100A5 Antibody |
| Antibody SKU: | CAB14779 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A5 (NP_002953.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100A5 (NP_002953.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | METP LEKA LTTM VTTF HKYS GREG SKLT LSRK ELKE LIKK ELCL GEMK ESSI DDLM KSLD KNSD QEID FKEY SVFL TMLC MAYN DFFL EDNK |
| Gene ID: | 6276 |
| Uniprot: | P33763 |
| Cellular Location: | |
| Calculated MW: | 10kDa/12kDa |
| Observed MW: | 11kDa |
| Synonyms: | S100A5, S100D |
| Background: | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain. |
| UniProt Protein Function: | S100A5: Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites. Belongs to the S-100 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cell soma; nucleus Molecular Function:protein homodimerization activity; copper ion binding; zinc ion binding; calcium ion binding |
| NCBI Summary: | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P33763 |
| NCBI GenInfo Identifier: | 62739837 |
| NCBI Gene ID: | 6276 |
| NCBI Accession: | AAH93957.1 |
| UniProt Secondary Accession: | P33763,Q5RHS3, |
| UniProt Related Accession: | P33763 |
| Molecular Weight: | 10,744 Da |
| NCBI Full Name: | S100 calcium binding protein A5 |
| NCBI Synonym Full Names: | S100 calcium binding protein A5 |
| NCBI Official Symbol: | S100A5 |
| NCBI Official Synonym Symbols: | S100D |
| NCBI Protein Information: | protein S100-A5; S100 calcium-binding protein A5 |
| UniProt Protein Name: | Protein S100-A5 |
| UniProt Synonym Protein Names: | Protein S-100D; S100 calcium-binding protein A5 |
| Protein Family: | Protein |
| UniProt Gene Name: | S100A5 |
| UniProt Entry Name: | S10A5_HUMAN |
 | Western blot analysis of extracts of U-251MG cells, using S100A5 Rabbit pAb (CAB14779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
 | Immunohistochemistry of paraffin-embedded human thyroid cancer using S100A5 antibody (CAB14779) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using S100A5 antibody (CAB14779) at dilution of 1:100 (40x lens). |
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